离子束辐照植物种子所产生的水自由基对植物种子存活率会有影响,实验中为了观察这种辐射效应,采用6.5MeV能量的质子分别辐照拟南芥干种子和含水种子。这个能量点的质子无论对干种子还是含水种子都可以完全穿透,减少了因损伤部位不同带来的差异。实验中采用的质子注量从4×10^9ions/cm^2到1×10^14ions/cm^2。实验结果显示干种子和含水种子存活曲线均成肩形下降趋势,而且含水种子下降注量点明显低于干种子。这是自由基作用结果。同时在辐射生物学靶学说的基础上,建立了模型,通过参数的合理设置,很好地拟合了实验数据。
The dry and water-saturated seeds of Arabidopsis thaliana were irradiated by H^+ ions with 6.5 MeV cm^2 . in atmosphere. The ion fluence used in this T experiment was in the range of 4 × 10^9-1 × 10^14 ions/ RIM simulation, the ions with the energy of 6.5 MeV can penetrate the whole seed. The experiment shows that the fluence-response curves for the dry seeds and water-saturated seeds had distinct shoulders and reduced rapidly. The experimental results show that the water-imbibed seeds were more sensitive than the dry seeds and the reason is from free radicals reaction. A model has been constructed, and primely simulates the experiment data.