中文摘要：

目的 探讨2型糖尿病（T2DM）周围神经病变（diabetic peripheral neuropathy,DPN）患者外周血中CD4＋CD25＋Foxp3＋调节性T细胞（Treg）的变化及其检测的临床应用。方法 采用流式细胞技术多色荧光分析法,对20例初诊老年2型DPN患者（DPN组）、20例初诊老年T2DM不伴并发症患者（T2DM组）及20例性别、年龄匹配的健康对照者（CON组）外周血中Treg细胞进行测定,对各组结果进行统计分析并进行比较。结果 与CON组相比,DPN组和T2DM组患者外周血CD4＋CD25＋Foxp3＋Treg细胞数量均减少（P〈0.05）,且DPN组少于T2DM组患者（P〈0.05）。DPN组患者外周血CD4＋CD25＋Foxp3＋Treg细胞比例与密歇根神经病变筛查量表（Michigan neuropathy screening instrument,MNSI）评分呈负相关,但无统计学意义;与密歇根糖尿病神经病变评分量表（Michigan diabetic neuropathy score,MDNS）评分负相关,有统计学意义（r=-0.339,P〈0.05）。结论 老年DPN患者外周血CD4＋CD25＋Foxp3＋Treg细胞数量减少,可能在DPN的发生、发展中起到一定作用。

英文摘要：

Objective To investigate the changes of CD4＋ CD25＋ Foxp3＋ regulatory T cell （Treg） in the peripheral blood of elderly patients with type 2 diabetic peripheral neuropathy （DPN） and its clinical implication. Methods Treg cells were detected by flow cytometry in the peripheral blood of elderly patients with type 2 DPN （DNP group, 20 cases）, type 2 diabetes mellitus （T2DM） with no complications （T2DM group, 20 cases） and age-matched healthy volunteers （CON group, 20 cases）. The results were statistically analyzed and compared between different groups. Results The numbers of CD4＋ CD25＋ Foxp3＋ Treg in DPN group and T2DM group were significantly less than that in the healthy volunteers （P〈0. 05）, and the number of Treg cells in DPN group was significantly less than that in T2DM group （P〈0.05）. The proportion of CD4＋ CD25＋ Foxp3＋ Treg was negatively associated with Michigan neuropathy screening instrument （MNSI） scores in DPN group, but with no statistical significance; however, the proportion had a significantly negative correlation with Michigan diabetic neuropathy score （r：--0. 339, P〈0.05）. Conclusion The number of CD4＋ CD25＋ Foxp3＋ Treg is decreased in the peripheral blood of elderly patients with DPN, which may play a role in the development and progression of DPN in elderly patients.