中文摘要：

为建立一套适合茶树雌蕊总蛋白质分离的双向电泳体系，对茶树雌蕊总蛋白的提取方法、SDS-PAGE凝胶浓度、聚焦条件和IPG胶条pH范围等进行了比较优化。结果表明，TCA/丙酮-clean up kit法提取总蛋白，用17 cm pH 5～8 IPG胶条，13%SDS-聚丙烯酰胺凝胶进行分离，用考马斯亮蓝R250染色，可以获得清晰、分辨率高、重复性好的双向电泳图谱，共检测到约1200个蛋白点，主要分布在pH 5～8，相对分子量14～117 kD范围内，碱性蛋白得到有效分离，可以满足茶树雌蕊的蛋白组学分析和研究。

英文摘要：

In order to establish a proper two-dimensional gel electrophoresis （2-DE） protocol for proteomic study of tea plant pistils, several protein extraction methods, density of SDS-PAGE gel, IEF procedures and pH gradient of IPG strip were compared. The results showed the optimized system includes： total proteins of sample extracted using TCA/acetone method followed with clean up, separating the proteins with 17 cm pH 5～8 IPG strips, 13%SDS-PAGE, and CBB R-250 stain method. About 1 200 spots can be detected, and these proteins mainly distributed in the pH 5～8, MW 14～117 kD range. With good separation of the basic protein, this will be helpful to proteomic research in tea plant.