中文摘要：

目的：研究癫痫儿童拉莫三嗪（LTG）唾液与血清浓度的相关性。方法：在取得患儿及其家长知情同意的情况下，收集我院诊断为原发性或无需特殊病因治疗的症状性癫痫患儿，达稳态血药浓度后采集肘静脉血1ml，并同时收集自然分泌状态下的唾液0．5ml。使用反相高效液相色谱法（RP-HPLC）对血清及唾液浓度进行测定，数据分析采用统计学软件SPSS11．5。结果：以LTG浓度（C）、LTG与内标的峰面积比（Y）作线性回归，分别得到血清浓度与唾液浓度的回归方程：Y=0．1824C-0．0080，r=0．9998；Y=0．1816 C-0．0119，r=0．9997。共收集39例癫痫患儿的53个血清及唾液浓度样本，其中男27人，女12人，平均年龄7．86岁（1～15岁）。LTG血清浓度（x）和唾液浓度（y）之间的相关性如下：y=0．5607 x-0．6074（n=53，r=0．9510，P〈0．001）。LTG唾液与血清浓度的比值为0．42±0．11。结论：LTG血清和唾液之间有显著的相关性，可以用唾液替代血液对LTG进行治疗药物监测，由于唾液的采集简便无创，尤其适用于儿童患者。

英文摘要：

AIM： To study the correlation of LTG concentrations between saliva and serum in children with epilepsy. METHODS： Mter informed consent was obtained from the parents or guardian of children, children with primary epilepsy were collected in Peking University First Hospital. When reaching the steady drug concentration, 1.0 ml blood samples were obtained by phlebotomy, and 0.5 ml saliva sample were collected at the same time. Serum and salivary LTG concentrations were determined by reversed-phase high performance liquid chromatography （RP-HPLC）. Statistics software SPSS11.5 was used for data analysis. RESULTS：Linear regression analysis was made by LTG concentration （C） and the peak area ratio （Y） of LTG vs. internal standard （flurazepam）, and the regression equation of serum concentration and saliva concentration were Y = 0. 1824C - 0.0080, r =0.9998 and Y = 0. 1816C - 0.0119, r = 0.9997, respectively. We collected 39 patients and 53 serum and saliva concentrations samples, of whom, 27 were male and 12 female, aged 1 to 15 years （the average was 7.86）. The correlation between LTG serum concentration （ x ） and saliva concentration （ y ） was y = 0. 5607 x - 0.6074 （n=53, r=0.9501, P〈0.001）. The mean saliva/serum LTG concentration ratio was 0.42 ± 0.11. CONCLUSION： A significant positive correlation was found between LTG serum and saliva concentrations. Saliva sample may be a useful alternative to serum for therapeutic monitoring of LTG treatment, because saliva collection is simpler, less painful and cheaper than blood drawing, thus children may particularly benefit from this method.