中文摘要：

把减少性的 cDNA 图书馆基于在一条根部分克隆定序，分享高类似到一个米饭 C2H2 锌手指抄写因素(ZFP15 ) 的一个表示顺序标签(EST ) 在小麦被获得。通过生物信息学途径，小麦 C2H2 类型 ZFP 基因指了 TaZFP15 被识别了并且描绘。作为 670 bp 的全身的 cDNA， TaZFP15 有 408 bp 的一个开的读物框架并且编码 135-aa 多肽。TaZFP15 包含二个 C2H2 锌手指领域，每一个有保存主题 QALGGH。典型 L 盒子，通常在 C2H2 类型抄写因素识别了，也在 TaZFP15 被发现了。种系发生的分析建议 TaZFP15 与米饭 ZFP15 分享高类似(GenBank 同意没有 AY286473 ) ，玉米 ZFP (GenBank 同意没有 NM_001159094 ) 并且在植物种类的另外的锌手指抄写因素基因的一个子集。TaZFP15 的表示由 starved-Pi 应力是起来调整的，显示一个模式逐渐地与在 23-h 处理政体的 Pi 压力的前进一起被提高。同样，在根的 TaZFP15 的抄本被干旱和咸度的氮缺乏，和不能生活的压力也导致。TaZFP15 的回答都没在根被检测到营养缺乏 P， Zn，和 Ca，和 abscisic 酸(骆驼毛的织物) 的外部处理。TaZFP15 能明确地在染色体 A， B，和 D 被放大，并且没有在序列的可变性，建议那 TaZFP15 在相应 hexaploid 种类有多拷贝。在烟草的转基因的分析表明 TaZFP15 的起来规定能显著地改进植物经由在 Pi 缺乏状况下面增加植物磷获得能力的干燥集体累积。结果建议 TaZFP15 涉及多样的外部压力的信号 transductions 的调停。

英文摘要：

Based on sequencing of part clones in a root subtractive cDNA library, an expressed sequence tag （EST） sharing high similarity to a rice C2H2 zinc finger transcription factor （ZFP15） was obtained in wheat. Through bioinformatics approach, the wheat C2H2-type ZFP gene referred to TaZFP15 has been identified and characterized. As a full-length cDNA of 670 bp, TaZFP15 has an open reading frame of 408 bp and encodes a 135-aa polypeptide. TaZFP15 contains two C2H2 zinc finger domains and each one has a conserved motif QALGGH. The typical L-box, generally identified in the C2H2 type transcription factors, has also been found in TaZFP15. Phylogenetic analysis suggested that TaZFP15 shares high similarities with rice ZFP15 （GenBank accession no. AY286473）, maize ZFP （GenBank accession no. NM_001159094） and a subset of other zinc-finger transcription factor genes in plant species. The expression of TaZFP15 was up-regulated by starved-Pi stress, showing a pattern to be gradually elevated along with the progression of the Pi-stress in a 23-h treatment regime. Similarly, the transcripts of TaZFP15 in roots were also induced by nitrogen deficiency, and abiotic stresses of drought and salinity. No responses of TaZFP15 were detected in roots to nutrition deficiencies of P, Zn, and Ca, and the external treatment of abscisic acid （ABA）. TaZFP15 could be specifically amplified in genome A, B, and D, and without variability in the sequences, suggesting that TaZFP15 has multi-copies in the homologous hexaploid species. Transgenic analysis in tobacco revealed that up-regulation of TaZFP15 could significantly improve plant dry mass accumulation via increasing the plant phosphorus acquisition capacity under Pi-deficiency condition. The results suggested that TaZFP15 is involved in mediation of signal transductions of diverse external stresses.