中文摘要：

苏远7235是我国利用异常棉等多个野生种创造的高纤维强力棉花种质系，是开展棉花纤维品质研究的重要材料。本研究以pIndigoBAC-5（HindⅢ—cloning ready）为载体，构建了苏远7235的细菌人工染色体（Bacterial Artificial Chromosome，BAC）文库，该文库包含30336个BAC克隆。分析结果表明，重组克隆苏远7235DNA插入片段为50—140kb，平均120kb，空载率2．1％，89．6%的克隆插入片段大于100kb。

英文摘要：

In this study, bacterial artificial chromosome （BAC） library of Suyuan7235, a cotton germplasm with high fiber strength has been constructed following the partial digestion of genomic DNA with HindⅢ. The pIndigoBAC-5 （HindⅢ-cloning ready） cloning vector was used for the library. The BAC library included 30336 clones. Analysis of 96 recombinants showed that the insert DNA size ranged from 50 to 140 kb, averaged 120kb with less than 2.1% of empty clones. As much as 89.6% clones had inserts over 100 kb. As genomic resources, the library has potential use in further research of fiber strength gene analysis and gene cloning, as well as SSR primer development.