采用DPPH·法及Fe3+还原力法对蛇菰提取物的抗氧化活性进行研究,分别测定蛇菰乙醇提物、石油醚提取物、氯仿提取物、乙酸乙酯提取物、正丁醇提取物、水层部分、水提粗多糖及碱提粗多糖的抗氧化作用,同时以VC作为阳性对照。结果表明:蛇菰提取物具有较强的抗氧化活性,尤其是乙酸乙酯提取物、正丁醇提取物、总醇提取物及水提粗多糖;它们对DPPH·的清除作用与VC相当,其中乙酸乙酯提取物IC50值为6.0μg/mL,活性略高于VC,同时以上提取物对Fe3+也具有很强的还原能力。
DPPH· free radical scavenging assay and ferric reducing power assay in vitro were used to evaluate the antioxidant activities of ethanol extract, petroleum ether extract, chloroform extract, ethyl acetate extract, n-butanol extract, water extraction fraction, water-soluble crude polysaccharides and alkaline-soluble crude polysaccharides from Balanophora spicata Hayata. Results indicated that the extracts from Balanophora spicata Hayata all exhibited strong scavenging capacity against DPPH free radicals, especially ethyl acetate extract, n-butanol extract, ethanol extract and water-soluble crude polysaccharides, which were nearly equivalent to vitamin C for DPPH free radical scavenging activity. The fact that ethyl acetate extract exhibited an IC50 value of 6.0 μg/mL suggested that a little stronger antioxidant activity of ethyl acetate extract than vitamin C. Meanwhile, the above extracts all revealed stronger ferric reducing power.