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藏药二十味沉香丸对大鼠低氧性肺动脉压力升高的干预作用
  • ISSN号:1001-4454
  • 期刊名称:《中药材》
  • 时间:0
  • 分类:R285.5[医药卫生—中药学;医药卫生—中医学]
  • 作者机构:[1]青海大学医学院,青海西宁810001
  • 相关基金:国家自然科学基金资助项目(30960522)
中文摘要:

目的:观察藏药二十味沉香丸对大鼠低氧性肺动脉压力升高干预作用,从而证实ET-1参与低氧性肺动脉高压的形成。方法:将165只Wistar大鼠随机分为高原对照组、二十味沉香丸藏药组和平原对照组,于给药后第1、3、7、15、30天用生理信号采集系统记录肺动脉压并计算左右心室质量比,用ELISA方法测定血清HIF-1α和ET-1蛋白水平,用Western Blot方法测定肺组织ETA受体水平。结果:与高原对照组比较,藏药二十味沉香丸组动物肺动脉压自给药后第7天显著降低(P〈0.01),自第3天左右心室比及血清HIF-1α含量显著降低(P〈0.05或P〈0.01),第3、7天血清ET-1含量显著降低(P〈0.05或P〈0.01),各时间点肺组织ETA受体表达均显著降低(P〈0.01或P〈0.001)。结论:ET-1是引起大鼠肺动脉压升高和右心室壁增厚的重要因素之一,其只在低氧早期发挥作用,并不参与低氧后期的肺动脉压升高。藏药二十味沉香丸能够防止大鼠肺动脉压过度升高和右心室壁增厚,其机制可能与直接抑制ET-1和ETA受体水平或抑制HIF-1α后间接引起ET-1水平下调有关。

英文摘要:

Objective: To investigate the role of Tibetan medicine-Twenty Wei Chenxiang Pill interfering with serum ET-1 level,in order to confirm that ET-1 is involved to the pathogenesis of hypoxic pulmonary hypertension. Methods: 165 Wistar rats were randomly divided into high altitude control group,Tibetan medicine-Twenty Wei Chenxiang Pill group and plain control group. The physiological signal acquisition system was used to record pulmonary arterial pressure,and RV /( LV + S) ratio were caculated. Serum HIF-1α and ET-1 protein levels were determined by the method of ELISA,and ETA protein levels in lung tissue were determined by Western Blot method. Results: Compared with the high altitude group,in the rats of Tibetan medicine-Twenty Wei Chenxiang Pill group,the pulmonary arterial pressure decreased significantly from the seventh day and the seventh day( P〈0. 01),the RV /( LV + S) ratio and serum HIF-1αlevels decreased significantly from the third day( P〈0. 05 or P〈0. 01),the serum ET-1 levels decreased significantly from the third day( P〈0. 05 or P〈0. 01),and the expression of ETA protein decreased significantly from the beginning( P〈0. 01 or P〈0. 001).Conclusion: ET-1 is one of the important factors causing pulmonary artery pressure increasing and right ventricular wall thickening,which plays a role in hypoxic pulmonary artery only involved in the early period hypoxia,but not in the later period. Tibetan medicinetwenty Wei Chenxiang Pill can prevent the pulmonary artery hypertension and the right ventricular wall thickening in rats,and its mechanism may be related to the direct inhibition of ET-1 and protein levels of ETA or the indirect downregulation of ET-1 level and ETA through inhibition of HIF-1α level.

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期刊信息
  • 《中药材》
  • 中国科技核心期刊
  • 主管单位:国家食品药品监督管理局
  • 主办单位:国家食品药品监督管理局中药材信息中心站
  • 主编:元四辉
  • 地址:广州市中山二路24号中粤大厦10楼
  • 邮编:510080
  • 邮箱:
  • 电话:020-81888465 81889570 87665465
  • 国际标准刊号:ISSN:1001-4454
  • 国内统一刊号:ISSN:44-1286/R
  • 邮发代号:
  • 获奖情况:
  • 全国医药信息成果二等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),荷兰文摘与引文数据库,美国生物医学检索系统,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:54900