中文摘要：

研究了污泥厌氧发酵过程中产甲烷菌被特异性抑制剂——2-溴乙烷磺酸盐（BrCH2CH2SO3^-，BES）抑制时乙酸的累积，并采用一种新的微生物分子生态学手段——末端限制性片段长度多态性分析（T—RFLP）研究乙酸累积状态下的微生物种群结构．结果表明，BES可以促使厌氧发酵中乙酸的累积，在污泥厌氧发酵初始时一次投加BES条件下，乙酸累积的最大值为1．158g L^-1，是阴性对照样最大值的4倍．BES在d4开始投加并每间隔4d补加条件下，乙酸累积最大值为0．851g L^-1．微生物种群结构分析表明，对于发酵初始投加BES的样品，乙酸累积主要来源于β-Proteobacteria和Sphingobacteria的作用．对于每4d投加BES的样品，Sphingobacteria在中间过程的d8占有较高比例，Bacilli和β-Proteobacteria在整个过程均占优势．图2表2参14.

英文摘要：

The accumulation of acetate and variation of microbial community structure during anaerobic fermentation of sludge were studied by using a special inhibitor-2-bromoethanesulfonate （BrCH2CH2SO3^- , BES） to inhibit methanogens. When BES added at the beginning of fermentation, it was found that BES could cause acetate to accumulate up to the highest concentration （ 1. 158 g L^-1 ） , which was four times as much as the control. On the other hand, acetate reached 0.851 g L^-1 when BES was added on the fourth day and supplemented every four days. The analysis of microbial community structure in sludge samples by terminal restriction fragment length polymorphism （T-RFLP） indicated that the accumulation of acetate was mainly due to the presence of both β-Proteobacteria and sphingobacteria. Referring to the samples with BES added on the fourth day and supplemented every four days, sphingobacteria occupied a relatively large proportion only on the eighth day, while bacilli and β-Proteobacteria were dominant throughout the whole process. Fig 2, Tab 2, Ref 14 .