中文摘要：

采用核磁共振波谱技术（NMR）研究小檗碱与牛血清白蛋白（BSA）的相互结合作用。比较不同浓度小檗碱在一定浓度的BSA溶液中的化学位移、弛豫时间和扩散系数的变化情况。研究表明,小檗碱环上芳香质子与BSA的结合作用较强,其它烷基质子与BSA结合作用较弱;BSA的结合使得小檗碱各个质子的1 HT1弛豫时间明显减少,质子在溶液中运动受限;小檗碱与BSA相互作用的饱和浓度之比为60∶1;根据自扩散系数拟合可以得到两者解离常数为0.44mmol/L;结合位点数为22。本研究为临床安全、合理使用小檗碱,以及开发改良小檗碱类似药物提供实验依据。

英文摘要：

1H nuclear magnetic resonance chemical shift,relaxation time and diffusion coefficient measurements were carried out to study the interaction on the affinity binding of Berberine,an antibacterial drug,to bovine serum albumin（BSA）.The results showed that the protons of aromatic ring of Berberine were more favorable to form Berberine-BSA complex than those of aliphatic groups.BSA combination resulted in significantly decrease in the relaxation time of Berberine protons,which suggests that the motion of these protons were limited in aqueous solutions.The saturation concentration ratio of Berberine to BSA is 60 ∶ 1 according to the measurements of chemical shift and relaxation time.The apparent dissociation constant was 0.44 mmol/L and the binding sites of Berberine molecules on BSA was 22 fitted from the self-diffusion measurements.This study is of great importance for the safe and rational use of Berberine in clinic,and development of improved Berberine analogs.