中文摘要：

研究溶瘤腺病毒SG511携带由热激蛋白70（hsp70）启动子调控IL-24构成的增殖型溶瘤腺病毒SG511-pHSP70-IL24对人肝癌细胞株的体外杀伤效果。实验采用ELISA法检测不同MOI值感染肝癌细胞SMMC-7721和Hep3B细胞3 d后IL-24的表达;MTT法检测SG511、Ad11-IL24以及SG511-pHSP70-IL24对肝癌细胞株SMMC-7721、Hep3B以及人正常成纤维细胞株MRC-5及BJ增殖的抑制作用;并通过结晶紫实验检测进一步证明联合用药对肿瘤细胞的杀伤情况。ELISA检测到SG511-pHSP70-IL24感染SMMC-7721和Hep3B细胞后,IL-24有明显表达;MTT实验结果表明SG511-pHSP70-IL24对肿瘤细胞的杀伤能力比SG511和Ad-IL24更强。同时结晶紫实验也证明了SG511-pHSP70-IL24对肿瘤细胞的杀伤能力比SG511和Ad-IL24更强。增殖型溶瘤腺病毒SG511-pHSP70-IL24相比腺病毒Ad11-IL24以及SG511,对肝癌细胞有较明显的生长抑制和凋亡诱导作用。

英文摘要：

The experiment is aimed at studying the anti-tumor effect of SG511-pHSP70-IL24 in hepatoma cells.First,ELISA experiment shows that IL-24 is expressed in Hep3B and SMMC-7721.Second,MTT assay is used to test the growth inhibition effects of single or combination therapy on tumor cell lines SMMC-7721,Hep3B and human normal cell line BJ and MRC-5.Cytopathic effect assay then is conducted to further demonstrate the efficacy of the combination therapy.Results show that the anti-tumor efficacy of SG511-pHSP70-IL24 has greater toxicity in killing Hep3B and SMMC-7721,showing no toxicity against normal cell line BJ and MRC-5.