中文摘要：

目的研究细胞因子IL-2、IL-15对人外周血来源的自然杀伤细胞（Nature Killer,NK）细胞表面功能相关分子杀伤细胞免疫球蛋白样受体（Killer Cell Immnoglobulin-like Receptor,KIR3DL1）、NKG2D表达的影响。方法采用miniMACS免疫磁珠从外周血单个核细胞（PBMNC）分选得到纯化的NK细胞,在IL2、IL15作用下培养15d,检测培养前后在不同效靶比下对靶细胞（K562）的杀伤作用变化,之后流式细胞仪检测培养前后NK细胞KIR3DL1、NKG2D表达变化;结果经IL2、IL15培养15d后的NK细胞对K562细胞的杀伤率在不同效靶比下均较培养前增强（P〈0.01）;同时NK细胞KIR3DL1表达下降约10%（P〈0.05）,NKG2D在培养前后均高表达（99%）;结论经IL2和IL15长期体外培养后,KIR3DL1表达轻微下调,是NK细胞杀伤功能增强的原因之一。

英文摘要：

Objective To study the effect of IL-2 and IL-15 on expression of KIR3DL1 and NKG2D in human peripheral blood derived natural killer（NK） cells.Methods NK cells were isolated from PBMNC using mini-magnetic cell-selection（MACS） and NK cell isolation kit（Miltenyi Biotec,Germany）,and cultured with stem cell growth medium（SCGM） supplemented with 5% human AB serum in combination with of IL-2 and IL-15 for 15 days.Cytotoxicity to target K562 cells was evaluated,and expression of KIR3DL1（CD158e） and NKG2D in NK cells was analyzed by flow cytometry.Results The cytotoxicity of NK cells was significantly higher after culture than before culture（P〈0.01）.The expression rate of KIR3DL1 in NK cells was about 10% lower after culture than before culture（P〈0.05）.No significant difference was found in expression of NKG2D,which was 99% before and after culture.Conclusion The cytotoxicity of NK cells after a long time in vitro culture with IL2 and IL15 significantly increases and down-regulates the expression of KIR3DL1 .