统合生物加工过程(Consolidated bioprocessing,CBP)具有应用于纤维素乙醇生产的潜力,而该技术的关键是构建能有效降解纤维素的工程菌株。酿酒酵母是传统的乙醇发酵菌株,作为CBP宿主菌株具有很多优势,因此在酿酒酵母中表达纤维素酶引起研究者的普遍关注。综述了纤维素酶基因在酿酒酵母中表达的影响因素,包括基因表达盒表达元件(启动子、信号肽和终止子等)、纤维素酶基因拷贝数及存在形式以及纤维素酶基因来源等,并对一种和多种纤维素酶基因在酿酒酵母中的表达及构建得到的CBP菌株研究进展做了简要介绍。
Consolidated bioproeessing (CBP) is a promising technology for lignocellulosic ethanol production, and the key to CBP is the engineering of a microorganism that can efficiently utilize cellulose. Saccharomyces cerevisiae is a traditional ethanol producing strain, and it has many advantages as the CBP host strains, so the expression of cellulase in S. cerevisiae is causing great interest. The factors that influence the expression of cellulase genes in S. cerevisiae, including gene expression cassette expression elements (promoter, signal peptide and terminator, etc. ), cellulase gene copy number and existing forms, the sources of cellulase gene and so on were reviewed. In addition, research progress about one or more cellulase genes expressed in S. cerevisiae and the construction of CBP strains were briefly introduced.