中文摘要：

目的研究敌敌畏（O，O-dimethyl-O-2，2-dichlorovinvlphosphate，DDVP）对人神经母细胞瘤细胞（SK-N-SH）的氧化损伤作用。方法MEM培养基培养SK-N-SH细胞，细胞计数测定SK-N-SH细胞群体倍增时间；不同浓度（0～120μmol／L）DDVP作用sK细胞48h后，MTT法测定SK-N-SH细胞48h半数抑制浓度（IC50）；巴氏染色后观察细胞形态变化；同时分别检测细胞内超氧化物歧化酶（SOD）活性、过氧化氢酶（CAT）活性、丙二醛（MDA）含量的变化。结果SK-N-SH细胞群体倍增时间（36．28±1．46）h；48h半数抑制浓度（IC50）为（105．22±5．80）pmlol／L：随着DDVP染毒浓度的增高，细胞内MDA的含量逐渐增高，SOD、CAT活性逐渐降低，呈现明显的剂量-效应关系（P〈0．05）。结论DDVP对SK-N-SH细胞有明显的氧化损伤作用。

英文摘要：

Objective To study the oxidative damage of O,O-dimethyl-O-2,2-dichlorovinylphosphate （DDVP） to human neuroblastoma SK-N-SH cells. Methods SK-N-SH cells were cultured by minimum essential medium, and counted to deter- mine the population doubling time. The SK-N-SH cells were treated with DDVP at different concentrations （0-120μmol/L） for 48 hours, and then the half maximal inhibitory concentration （ IC50 ） was measured by MTT assay. Cell morphological changes were observed under light microscopy by Giemsa staining; meanwhile, the activity of superoxide dismutase （ SOD ） and catalase （ CAT ） as well as intracellular malondialdehyde /MDA） content were detected. Results The population doubling time of SK-N-SH cells was （36.28±1.46） hours. The IC50 of DDVP treatment for 48 hour in SK-N-SH cells was （105.22±5.80） μmol/L. With the increased concentration of DDVP, intracellular MDA content gradually increased, while the activity of SOD and CAT gradually de- creased, showing a significant dose-response relationship （P〈0.05）. Conclusions DDVP can cause oxidative damage to hu- man neuroblastoma SK-N-SH cells.