中文摘要：

目的在Tet-on-LMPlHNE2鼻咽癌细胞中探讨EB病毒编码的潜伏膜蛋白1（LMP1）调控p16和cyclinD1的转录．方法采用Westernblot方法、荧光素酶报道系统和RT-PCR方法检测在DOX诱导下不同时间点p16和cyclinD1的启动子活性和转录．结果LMP1可下调p16启动子活性和mRNA表达；同时上调cyclinD1启动子活性和mRNA表达．结论 在Tet-on-HNE2鼻咽癌细胞中EB病毒LMP1在转录水平调控G1／S检测点重要相关蛋白p16和cyclinD1的表达．

英文摘要：

Objective To investigate on EB encoding latent membrane protein 1 （LMP1） regulating the transcription of p16 and cycin D1 in the Tet-on-LMP1 HNE2 nasopharyngeal carcinoma cell line. Methods p16 and cyclin D1 promoter activity and transcription with DOX induction were detected by western blot assay, luciferase reporter system and RT-PCR at the different timepoint. Results LMP1 downregulated p16 promoter activity and mRNA expression and upregulated cyclin D1. Conclusion EB LMP1 can regulate G1/S checkpoint significantly-related p16 and cyclin D1 expression at the transcriptional level in the Tet-on-LMP1 HNE2 nasopharyngeal carcinoma cell line.