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中文摘要：
目的：探讨白细胞介素-34/集落刺激因子-1受体（IL-34/CSF-1R）在转化生长因子-β1（TGF-β1）诱导A549细胞发生上皮-间质转化（ EMT）中的表达。方法体外培养A549细胞；CCK 8法检测不同浓度TGF-β1在不同时间点对A549细胞增殖的影响；选用5μg/L TGF-β1刺激A549细胞（0、12、24、48h），提取细胞蛋白和RNA， Western blotting法检测α-平滑肌肌动蛋白（α-SMA）、E-钙黏连蛋白（E-Cad）、基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（ MMP-9）、基质金属蛋白酶抑制剂-1（ TIMP-1）蛋白的变化；Real-time PCR 法检测 IL-34、CSF-1R、MMP-2、MMP-9基因表达的变化。结果 TGF-β1对A549细胞的增殖无明显影响（ P＞0.05）。 TGF-β1刺激A549细胞后，间质细胞标志性蛋白α-SMA表达升高，上皮细胞标志性蛋白E-Cad表达下降，纤维化相关的MMP-2蛋白表达升高，MMP-9蛋白表达先升高后下降，TIMP-1蛋白早期出现短暂性增高后，呈持续降低趋势（ P＜0.01）。 IL-34 mRNA表达逐渐升高，CSF-1R mRNA、MMP-2 mRNA、MMP-9 mRNA表达先升高后下降（P＜0.01）。结论 TGF-β1诱导A549细胞的EMT过程中，存在IL-34/CSF-1R的动态表达。
英文摘要：
Objective To investigate the expression of interleukin （IL）-34/colony stimulating factor（CSF）-1R in the process of transforming growth factor （ TGF）-β1 inducing epithelial-mesenchymal transition （ EMT） of human alveolar epithelial cells A549 cells.Methods A549 cells were cultured in vitro.CCK 8 was used to test the influence of the proliferative rate of A549 cells which were stimulated by TGF-β1 at different concentrations and time points .A549 cells were stimulated by 5μg/L TGF-β1 at 0 hour, the 12th hour, the 24th hour, and the 48th hour.Western blotting was adopted to detect changes of the following proteins： α-smooth muscle actin （α-SMA ） , E-cadherin （ E-Cad ） , matrix metalloproteinase-2 （MMP-2）, matrix metalloproteinase-9 （MMP-9） and tissue inhibitor of metalloproteinase-1（TIMP-1）. Real-time PCR was adopted to detect changes of the following genes： IL-34 mRNA, CSF-1R mRNA, MMP-2 mRNA and MMP-9 mRNA.Results TGF-β1 had no significant influence in the proliferation of A 549 cells compared with the control group（P〉0.05）.TGF-β1（5μg/L）stimulated A549 cells at different time point （0 hour, 12, 24, 48 hours）, compared with the control group .The epithelial phenotype E-Cad protein was gradually down-regulated （ P 〈0.01 ） , while the mesenchymal phenotype α-SMA protein was gradually up-regulated （ P 〈0.01 ） and the protein of MMP-2 increased gradually （P〈0.01）.The protein of MMP-9 increased firstly and then was reduced （P〈0.01）,the peak was at the 24th＆amp;nbsp;hour.The protein of TIMP-1 was firstly transiently increased and then reduced （P〈0.01）, the minimum was at the 48th hour.Compared with the control group , the gene of IL-34 mRNA increased gradually （P〈0.01）, and the genes of CSF-1R mRNA, MMP-2 mRNA and MMP-9 mRNA increased firstly and then decreased （ P 〈0.01）, which were peaked respectively at the 24th hour, the 24th hour, the 12th hour, respectively.Conclusion In the process of TGF-β1 inducing A549 c
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