从吉林省抚松地区采集到的人参根际土壤中分离、纯化获得97株细菌,采用平板对峙法筛选出13株对毁灭柱孢菌Cylindrocarpon destructans (Zinss.) Scholtan具有良好抑制作用的菌株.用生长速率法对抑菌效果最好的菌株SZ-22进行抑菌谱的测定,并进行盆栽试验验证其对人参锈腐病的防治效果.抑菌试验结果表明,菌株SZ-22发酵液对毁灭柱孢菌的抑制率达82.63%,具有较好的广谱抑菌作用.盆栽试验结果表明,菌株SZ-22对人参锈腐病的防治效果为63.02%,显著高于农药多菌灵的防效.经形态特征、生理生化特征鉴定及16S rDNA序列分析,确定菌株SZ-22为土壤短波单胞菌Brevundimonas terrae.
Using the plate confrontation assay, thirteen strains with antagonistic effect on Cylindrocarpon destructans (Zinss.) Scholtan were screened out of 97 bacteria which were isolated from the ginseng rhizosphere soil of Fusong County of Jilin Province in China. The plate-confrontation method and growth speed rate method were used for preliminary screening and antimicrobial test in vitro. Pots trails were carried out to assess the inhibitory activity of strain SZ-22 in vivo. The results showed that the inhibition rate of strain SZ-22 fermentation broth was 82.63% to C. destructans (Zinss.) Scholtan and also had good broad-spectrum antimicrobial effect. The strain SZ-22 had significant control efficacy when compared with the pesticides treatments, the control efficacy of the aseptic filtrate of SZ-22 reached to 63.02% with no dilution, and more than 50% in 10 times dilution in pots trails, respectively. According tomorphologic, physiological and biochemical characteristics and 16S rDNA sequence analysis, strain SZ-22 was classified as Brevundimonas terrae.