欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
在弱酸性介质中，小分子迈格林华尔色素与生物大分子核糖核酸发生强烈相互作用，导致分子构象变化，进而引起吸收光谱最大波长和吸收值的变化。研究了缓冲溶液酸度、色素用量、反应时间对反应体系的影响，确定了最佳实验条件，建立了测定核糖核酸的新方法。方法的线性范围为0～8．0mg／L，相关系数为0．9996，直接测定样品中的核糖核酸含量，结果满意。该文还初步探讨了反应机理，认为静电缔合作用使迈格林华尔色素分子中的正电荷与RNA分子链上的反离子交换，然后以协同方式与RNA分子发生键合，使色素π-电子叠合程度降低，颜色变浅，吸收值降低，最大吸收波长紫移。
英文摘要：
Based on the fact that both the maximum wavelength of the absorption spectrum of May Grunwald＇s stain（MGS） and the corresponding absorbance varied when MGS interacted with the large biological molecules of RNA under weak acidic medium, a novel spectrophotometric method for the determination of RNA was proposed. The effects of the acidity of B - R buffer solution, the amount of MGS, the amount of B - R buffer and the time on the interaction of the system were studied in detail and optimized. The decreased absorbance of MGS has a linear relationship with the content of RNA in the range of 0 -0. 8 mg/L and the correlation coefficient was 0. 999 6. The method is simple, rapid, highly sensitive and selective. It was applied for the determination of RNA in synthetic samples with satisfactory result. The reaction mechanism was preliminary studied.
同期刊论文项目
同项目期刊论文
Synthesis and Characterization of Nano-Crystals Molecular Hybrid Material with 3,3’,5,5’-Tetramethyl
Photoinduced Electron Transfer and Electrocatalytic Properties of Novel Charge-Transfer Compound (TM
A one-step method to construct a third-generation biosensor based on horseradish peroxidase and gold
An Exercise Degree Monitoring Biosensor Based on Electrochemiluminescent Detection of Lactate in Swe
Disposable superoxide anion biosensor based on superoxide dismutase entrapped in silica sol–gel matr
Study on Sensitization from Reactive Oxygen Species for Electrochemiluminescence of Luminol in Neutr
Electrodeposition of large size gold nanoparticles on indium tin oxide glass and application as refr
Electrodeposition of gold nanoparticles on indium/tin oxide electrode for fabrication of a disposabl
Study on a Pyruvate Oxidase Biosensor Based on β-Cyclodextrin Included Ferrocene as Electron- transf
One-step method embedding superoxide dismutase and gold nanoparticles in silica sol-gel network in t
A carbon nanotube/silica sol-gel architecture for immobilization of horseradish peroxidase for elect
Direct electrodeposition of gold nanoparticles onto indium/tin oxide film coated glass and its appli
Spectrophotometric determination of deoxyribonucleic acid by its quenching effect on acridine orange
A reusable electrochemical immunosensor for carcinoembryonic antigen via molecular recognition of gl
Studies on sensitization for electrochemiluminescence of luminol with platinum-gold bimetallic nanop
期刊信息
位置：