以切花秋菊‘神马’为试材,研究了菊花花芽分化过程中叶片和顶芽中内源亚精胺(Spd)含量的变化规律,同时研究了外源Spd及其生物合成抑制剂二环己胺(DCHA)对菊花花芽分化的影响,以及其过程中叶片和芽内碳水化合物、蛋白质含量的变化规律。结果表明:菊花花芽分化起始期和小花原基分化期叶片中Spd含量较低,而在顶芽内大量积累Spd;短日照处理开始后,碳水化合物含量呈上升趋势,可溶性蛋白含量呈下降趋势;Spd处理提高了成花诱导过程中菊花叶片中碳水化合物和可溶性蛋白含量,促使可溶性蛋白提前达到最高值,促进花芽分化的进程;而DCHA处理得到与Spd处理相反的结果。
This paper studies the changing rule of endogenesis spermidine (Spd) content in autumn-cutting chrysanthemum 'Jinba' in apical buds and leaves during floral bud differentiation, meanwhile analyses the effects of Spd and dicyclohexylamine (DCHA) on the process of floral bud differentiation of ' Jinba' as well as the changing rule of carbohydrates and soluble protein content. The results showed that, high levels of Spd in apical buds was accumulated during the initiative stage of floral bud differentiation and the stage of floret primordia differentiation, while the levels of Spd in leaf were lower. After short day treatment, the content of carbohydrates increased and the content of soluble protein decreased. The content of carbohydrates and soluble protein were increased by exogenous Spd, promoting the soluble protein reached its highest levels in advance, then promoted floral bud differentiation. The DCHA treatment had the opposite result compared with Spd. These results provide a theoretical basis for the development of chrysanthemum.