中文摘要：

采用5-溴-4-氯-3-吲哚磷酸盐（BCIP）/氯化硝基四氮唑蓝（NBT）显色体系,构建了阵列纸芯片比色检测碱性磷酸酶（ALP）的方法。首先,借助烘干处理方式在光刻法制备的阵列纸芯片微孔中固定显色试剂,然后加入ALP进行显色反应,最后,采用凝胶成像仪和普通照相机成像,读取显色强度（灰度值）进行比色检测。详细考察了显色条件对检测结果的影响,探讨了人血清白蛋白对ALP检测的增色效应,在最佳实验条件下,ALP检测的线性范围为1.5~20 U/L,检出限（3#）为0.78 U/L（n=18）,比文献报道中纸芯片上检测ALP方法的检出限低约两个数量级。本方法成功用于实际血清样品检测,测定结果与临床值一致。在此基础上,构建了双色阵列纸芯片,通过颜色的变化实现了ALP的可视化半定量检测。

英文摘要：

A low-cost,simple and sensitive colorimetric detection method of alkaline phosphatase（ ALP） has been developed on microfluidic paper-based analysis devices. In a typical colorimetric detection,5-bromo-4-chloro-3-indolyl phosphate disodium salt（ BCIP） and nitro blue tetrazolium（ NBT） were firstly added onto the circle array zones of paper devices. Then ALP solutions were spotted to the array zones to perform the colorimetric reaction. The color results were recorded by both Gel Documentation systems and a common camera,and analyzed with Quantity One software. All the reaction conditions were aptimized. Under the optimal conditions,the colorimetric intensity showed a linear correlation to the concentration of ALP in the range of 1. 5 to 20 U / L with the limit of detection（ LOD） of 0. 78 U / L（ 3σ）,which was about two orders of magnitude lower than that of the reported method on μPADs. Besides,it could be applied in spiked real sample analysis with a satisfactory result. In addition,a dual-color array-based paper strip has been fabricated for the semiquantitative detection of ALP. The approximate activity of ALP could be simply distinguished by observing the color change with naked eyes.