中文摘要：

目的：探讨慢性阻塞性肺疾病（COPD）大鼠远端原代肺动脉平滑肌细胞（PASMCs）中单核细胞趋化蛋白-1（MCP-1）、转化生长因子-β1（TGF-β1）的分泌情况及其与Toll样受体4（TLR4）信号通路的相关性。方法：通过脂多糖（LPS）诱导建立大鼠COPD模型,分离培养、鉴定原代大鼠PASMCs,以LPS诱导PASMCs,然后分组（对照组、LPS组、TAK-242组,LPS＋TAK-242组）培养细胞,用Western blot法检测各组PASMCs中TLR4的表达,ELISA法检测细胞培养上清液MCP-1、TGF-β1的浓度,并进行相关性分析。结果：LPS组较对照组PASMCs中TLR4的表达水平上调（P〈0.05）,细胞培养上清液中MCP-1、TGF-β1的含量升高（P〈0.05）;TAK-242组PASMCs中TLR4的表达水平下调（P〈0.05）,上清液中MCP-1、TGF-β1与TLR4无相关性。结论：LPS可诱导大鼠原代PASMCs合成分泌MCP-1、TGF-β1,同时上调TLR4表达水平。

英文摘要：

Aim： To probe into the secretion of monocyte chemoattractant protein 1（ MCP-1）,transforming growth factor β1（ TGF-β1） in primary pulmonary artery smooth muscle cells（ PASMCs） of chronic obstructive pulmonary disease（ COPD） rats and the correlation with toll-like receptor 4（ TLR4） signaling pathways. Methods： The COPD rat model was established,and the PASMCs were separated,induced by LPS,and then divided into control group,LPS group,TAK-242 group,and LPS ＋ TAK-242 group. The expression of TLR4 in PASMCs of each group was detected by Western blot,and the concentrations of MCP-1 and TGF-β1 in cell culture fluid were determined by ELISA,and at last a correlation analysis was conducted. Results： In contrast with the control group,the expression of TLR4 in PASMCs of the LPS group was inecreased（ P〈0. 05） and the concentrations of MCP-1 and TGF-β1 in cell culture fluid were increased（ P〈0. 05）. The expression of TLR4 in PASMCs of the TAK-242 group was down-regulated（ P〈0. 05） and the concentrations of MCP-1 and TGF-β1 in cell culture fluid showed no correlation with the expression of TLR4. Conclusion： LPS can induce PASMCs of rats to synthesize and secrete MCP-1 and TGF-β1,and the TLR4 is up-expressed at the same time.