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中文摘要：
本文采用银染增强金纳米粒子（AuNPs）为信号因子，构建了一种新型的灵敏检测羟基自由基（·OH）的DNA电化学传感器．首先，巯基化的DNA1通过Au-S键自组装于金基底电极表面．然后．由Fenton反应产生的·OH可引起电极表面DNA1自组装层的氧化损伤裂解．未损伤的DNA1可与功能化AuNPs上的DNA2杂交．利用AuNPs对银离子的催化还原反应，将银原子沉积在AuNPs的周围，形成一层银外壳，再用差分脉冲伏安法（DPV）技术对沉积的银进行电化学检测，从而实现·OH的定量分析．研究结果表明，在最优实验条件下，该传感器检测·OH的线性范围为0．2～200μmol·L^-1，检测下限为50nmol·L^-1．该传感器有较好的重复性、选择性，并在抗氧化剂抗氧化能力评估方面具有潜在应用价值．
英文摘要：
A novel DNA-based electrochemical sensor has been successfully constructed for sensitive detection of hydroxyl radical （. OH） based on the silver-enhanced gold nanoparticle label. Thiolated DNA1 was firstly immobilized on the gold electrode through Au--S bonds. The .OH generated from Fenton reaction could induce serious oxidative damage of the DNA1 layer on the electrode surface. Then DNA2-functionalized gold nanoparticles （DNA2-AuNPs） were linked on the electrode through the hybridization between DNA2 and undamaged DNA1. Based on the catalytic reduction of silver ion by AuNPs, a silver layer was formed on the surface of AuNPs. The quantitative assay of-OH was carried out by differential pulse voltammetry （DPV） detection of the deposited silver. Under the optimization conditions, the developed DNA-based biosensor could detect · OH quantitatively with wide linear range （0.2 · 200 μmol. Lt） and low detection limit （50 μmol·L^-1）, and exhibited satisfactory selectivity and reproducibility. This electrochemical biosensor could have potential application in the evaluation of antioxidant capacity.
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