采用线粒体细胞色素b(Cyt b)为分子标记,研究了安徽长江流域黄鳝6个地理种群(当涂、无为、繁昌、贵池、怀宁和望江)共180尾个体的遗传变异关系。结果显示,在1087 bp序列中共检测到变异位点101个,单倍型68个,碱基组成中A+T的含量大于G+C的含量。6个地理种群的单倍型多样性(0.623~0.940)较高,核苷酸多样性(0.001 78~0.019 04)较低。群体分化指数(Fst:0.026 12~0.947 07)、基因流(Nm:0.027 94~18.6400)和分子变异分析(AMOVA)结果表明,安徽长江流域黄鳝一些地理种群间存在着明显的遗传分化。单倍型系统进化树和进化网络图表明:6个地理种群分为2个大的进化支,当涂与繁昌种群为一支,其余4个种群为另一支。地理隔离和黄鳝有限的迁移能力可能是造成种群遗传分化的原因。
180 samples of Monopterus albus were collected from 6 localities of Yangtze River basin in Anhui province, and their genetic structure was analyzed based on Cytochrome b (Cyt b). Of 180 Cyt b gene sequences (1087 bp), 68 haplo- types were identified with 101 mutation sites detected. The A + T content was significantly greater than G + C content in base composition. The haplotype diversity and nucleotide diversity of six populations were O. 623 -0. 940 and 0. 001 78 -0. 019 04, respectively. The genetic differentiation index (Fst), gene flow (Nm) and molecular variance (AMOVA) revealed sig- nificant genetic differentiations among populations. Neighbor-Joining tree and statistical parsimony network based on haplo- types indicated that DT and FC populations clustered together and other 4 populations formed a clade. It suggersted that the geographic isolation and limited migration ability of M. albus might contribute to the genetic differentiation.