中文摘要：

研究了Ni^2＋浓度对混合细菌发酵产氢的影响。结果表明,在反应温度为35℃、初始pH值为7.0的条件下,当Ni^2＋浓度为0～0.2 mg/L时可以提高混合细菌的产氢速率;当Ni^2＋浓度为0～0.1 mg/L时,随着Ni^2＋浓度的提高则累积产氢量和比产氢量均增大,其最大值分别可达288.6 mL和296.1 mL/g葡萄糖。当Ni^2＋浓度为0～50 mg/L时,液相末端产物主要为乙醇、乙酸和丁酸,没有检测出丙酸,且在该浓度范围内Ni^2＋对葡萄糖降解率的影响不大。当Ni^2＋浓度为0～0.1 mg/L时,随着Ni^2＋浓度的提高则生物量增加,最大比生物量为232.5 mg/g葡萄糖。当Ni^2＋浓度为0～50 mg/L时,反应结束时的pH值都比初始的低。

英文摘要：

The effect of the Ni^2＋ concentrations on fermentative hydrogen production by mixed cultures was investigated.The results show that at 35 ℃ and initial pH 7.0,the hydrogen production rate can be enhanced with increasing Ni^2＋ concentrations from 0 to 0.2 mg/L.The cumulative hydrogen yield and specific hydrogen yield increase with increasing Ni^2＋ concentrations from 0 to 0.1 mg/L,with the maximums being 288.6 mL and 296.1 mL/g glucose respectively.When Ni^2＋ concentration ranges from 0 to 50 mg/L,the major aqueous metabolites produced by mixed cultures are ethanol,acetic acid and butyric acid,no propionic acid is detected.Ni^2＋ concentration has little effect on the glucose degradation efficiency with increasing concentrations from 0 to 50 mg/L.The biomass increases with increasing Ni^2＋ concentrations from 0 to 0.1 mg/L and the maximum specific biomass yield of 232.5 mg/g glucose is obtained.The final pH value is lower than the initial pH with increasing Ni^2＋ concentrations from 0 to 50 mg/L.