中文摘要：

目的研究重楼总皂苷及不同重楼皂苷成分Ⅰ，Ⅱ，Ⅲ，Ⅴ，Ⅵ，Ⅶ，H对痤疮相关病原菌（痤疮丙酸杆菌、表皮葡萄球菌和金黄色葡萄球菌）的抑菌效果。方法通过液体微量稀释法检测重楼总皂苷及不同皂苷成分对痤疮相关病原菌的最小抑菌浓度（MIC）。结果重楼总皂苷对痤疮丙酸杆菌NCTC737，ATCC6919，表皮葡萄球菌ATCC12228，金葡菌ATCC6538的MIC分别为2．5，5．0，5．0，1．25mg／mL；重楼皂苷Ⅰ，Ⅱ，Ⅲ，Ⅴ，Ⅵ，Ⅶ，H对痤疮丙酸杆菌NCTC737的MIC分别为0．6，1．2，2．5，5．0，2．5，2．5，10．0mg／mL；对表皮葡萄球菌ATCC12228的MIC分别为1．2，1．2，5．0，5．0，2．5，2．5，10．0mg／mL；对金葡菌ATCC6538的MIC分别为0．6，0．6，2．5，5．0，2．5，2．5，10．0mg／mL。结论重楼总皂苷具有较好抑制痤疮相关病原菌的作用；重楼皂苷Ⅰ，Ⅱ，Ⅵ，Ⅶ在体外对痤疮发病相关菌具有明确的抑制作用，其中重楼皂苷Ⅰ的抑菌作用最强。

英文摘要：

Objective To study the inhibitory effect of total saponins, polyphylla Ⅰ,Ⅱ,Ⅲ,Ⅴ,Ⅵ,Ⅶ and H on acneassociated pathogens （Propionibacterium aches, Staphylococcus epidermidis and Staphylococcus aureus ）. Methods Liquid microdilution method was used to determine the minimal inhibitory concentration （MIC） of total saponins and its fractions on P. acnes ,S. epidermidis and S. aureus＇s. Results MICs of total saponins on Propionibacterium aches NCTC737, ATCC6919, Staphylococcus epidermidis ATCC12228, Staphylococcus aureus ATCC6538 were 2. 5,5.0,5.0,1.2mg/mL, respectively; MICs of polyphylla Ⅰ , Ⅱ, Ⅲ, Ⅴ, and H on Propionibacterium aches NCTC737 were 0. 6,1.2,2. 5,5,2. 5,2. 5,10. 0mg/mL respectively; MICs of polyphylla Ⅰ,Ⅱ,Ⅲ,Ⅴ,Ⅵ,Ⅶ and H on Staphylococcus epidermidis ATCC12228 were 1.2,1.2,5,5, 2. 5,2. 5,10. 0mg/mL respectively, while their MICs on Staphylococcus aureus ATCC6538 were 0. 6,0. 6, 2. 5,5,2. 5,2. 5,10. 0mg/mL respectively. Conelusion Total saponins of polyphylla can effectively inhibit acne-associated pathogens ; polyphylla Ⅰ , Ⅱ , Ⅵ and Ⅶ, in particularly polyphylla Ⅰ, also effectively inhibit acne-associated pathogens in vitro.