中文摘要：

目的评价等位基因特异性PCR（AS-PCR）检测白介素28B（IL28B）rs12979860基因多态性在预测丙型肝炎患者抗病毒治疗效果中的应用价值。方法选取2011年5月至2012年5月武汉大学人民医院感染科174例丙型肝炎住院患者外周血，针对rs12979860基因多态性设计特异性引物2对，并在反向引物3’末端第2或第3碱基处引入突变碱基，用AS-PCR检测30例丙型肝炎患者rs12979860基因多态性，并用基因测序法平行验证，比较2种检测方法的符合率、敏感度和特异度。然后采用AS-PCR方法分析174例丙型肝炎患者rs12979860不同基因型在人群中的分布频率。结果AS．PCR和测序法平行检测30例丙型肝炎患者，均能有效鉴别rs12979860基因的CC型、CT型和TT型，二者符合率为100％（X2=60．0，P〈0．01），特异度和敏感度也均为100％。以CC基因型为对照，AS-PCR检测TT基因型灵敏度为10-5，测序法为2×10-1。用AS-PCR法从174例丙型肝炎患者中，检出rs12979860基因的TT型、CC型和CT型的分布频率分别为3．5％（6／174）、13．2％（23／174）和83．3％（145／174）。结论AS-PCR法可快速、准确、可靠、经济、有效地检测丙型肝炎患者IL28Brs12979860基因多态性，适于各大医院预测丙型肝炎患者抗病毒治疗效果。（中华检验医学杂志，2013．36：59-62）

英文摘要：

Objective To evaluate the value of detection of interleukin 28B （IL28B） rs12979860 by allele-specific PCR （AS-PCR） for the prediction of antiviral treatment hepatitis C patients. Methods One hundred seventy-four blood samples were random collected from hospitalized patients with hepatitis C, who came from department of infectious diseases, Renmin Hospital of Wuhan University from May 2011 to May 2012. Two pairs of specific primers were designed for rs12979860 gene polymorphisms, and one mutated base was introduced to the second or third site of the end of 3＇ with the reverse primer, rs12979860 gene polymorphism of 30 cases with hepatitis C was detected by AS-PCR, and gene sequencing was further used to verify the consistency of the two methods in parallel. Then, the frequency distribution of different rs12979860 genotypes with 174 cases were analyzed by the AS-PCR method in the population. Results The genotype CC, CT or TT of rs12979860 with 30 cases could be well identified by both AS-PCR and gene sequencing, and the coincidence rate was 100% （X2 = 60. 0, P 〈 0. 01 ）. Compared to gene sequencing, both of the sensitivity and specificity of AS-PCR were 100%. Compared to the control （CC genotype）, 3T genotype detection sensitivity by AS-PCR was 10-5, while sequencing sensitivity was 2 × 10-1. rs12979860 polymorphism in the TT, CC and CT genotype distribution in the Chinese population frequencies were 3.45% （ 6/174 ）, 13.2% （ 23/174 ） and 83.3% （ 145/174 ）, respectively. Conclusion AS-PCR can quickly, accurate, reliable, economic and efficiently detect IL28B rs12979860 gene polymorphism of hepatitis C in patients, which could predict the effect of antiviral therapy on patients with hepatitis C. （ Chin J Lab Med, 2013,36：59-62）