中文摘要：

目的：研究青蒿琥酯（ART）对增生性瘢痕来源的成纤维细胞（HFBs）的Ⅲ型胶原（COLⅢ）蛋白表达的影响。方法：原代培养人HFBs,分为空白对照组和ART干预组（分别用75μmol/L、150μmol/L、300μmol/L、600μmol/L ART干预24h）,荧光定量PCR（q-PCR）法检测Ⅲ型前胶原α1链（proCOL3A1）的基因转录情况,western blotting法检测COLⅢ蛋白的表达,酶联免疫吸附试验（ELISA）检测基质金属蛋白酶1（MMP-1）的含量。结果：与空白对照组比较,ART干预组的proCOL3A1 mRNA相对表达量明显降低,且不同浓度ART组间比较差异有统计学意义（P〈0.01）。ART干预组COLⅢ蛋白表达水平明显下降,细胞培养上清液中MMP-1含量明显升高,且各浓度组间两两比较差异均有统计学意义（均P〈0.05或P〈0.01）。结论：ART能抑制人HFBs的COLⅢ的基因转录和蛋白水平,同时促进MMP-1分泌增加,加速胶原降解,抑制细胞外基质沉积,这可能是ART治疗增生性瘢痕的机制之一。

英文摘要：

Objective： To investigate the effect of artesunate （ART） on the expression of type Ⅲ collagen （COL Ⅲ） in hypertrophic scar fibroblasts （HFBs）. Methods： HFBs isolated from tissue specimens of hypertrophic scar were treated with different concentrations （75, 150, 300 and 600 μmol/L） of ART for 24 h. The mRNA expression of proCOL3A1 was detected by fluorescent quantitative PCR （q-PCR）. The protein expression of COL Ⅲ was detected by western blotting. The matrix metalloproteinase （MMP） 1 level in supernatants was assessed by enzyme-linked immuno-sorbent assay （ELISA）. Results： The pro- COL3A1 mRNA level and the COL Ⅲ protein expression level were decreased, while the MMP-1 level in supernatants of culture medium was increased in ART groups as compared with the control group （ P 〈 0.01）. There were statistical differences in above indexes among different ART concentration groups （ P 〈0.05 or P 〈0.01）. Conclusion： ART may have a curative effect on hypertrophic scar, and the mechanisms may be related to the inhibition of MMP-1 release and the regulation of COL Ⅲ expression.