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中文摘要：
【目的】通过对缺血再灌注早期脑组织的内皮型一氧化氮合酶（endothelial nitric oxide synthase，eNOS）表达情况的观察，探讨一氧化氯在脑缺血再灌注损伤早期发挥神经毒性作用时eNOS亚型的变化。【方法】采用线栓法制作大鼠脑缺血再灌注模型，免疫纽化及western blot方法检测缺血脑组织的eNOS蛋白表达变化。【结果】免疫组化及western blot结果显示缺血区脑组织血管内皮细胞内的eNOS表达在缺血1h内达高峰，缺血2h到再灌注2h内持续降低。【结论】大鼠脑缺血再灌注模型中血管内皮细胞eNOS出现变化，表明一氧化氮在缺血性脑损伤的病理过程的作用发挥与eNOS亚型的变化有关。
英文摘要：
[Objective] To explore the change of the subtypes of endothelial nitric oxide synthase（eNOS） while nitric oxide（NO） plays neurotoxic effect in the early stage of cerebral ischemia-reperfusion through observing the expression of eNOS in brain tissues of rats during cerebral ischernia-reperfusion. [Methods] Cerebral ischemia-reperfusion injury was induced by 2-hour suture occlusion of the unilateral middle cerebral artery, immediately after suture withdrawal to allow reperfusion. The eNOS expressions were examined by the method of immunohistochemistry and western blot. [Results] The eNOS expression increased in 1-hour during ischemia, keeping up with decreasing until reperfusion 2-hour. [Conclusion]The expression of eNOS in rats changes during cerebral ischemia-reperfusion. It indicates that the role of NO in pathogenesis of cerebral ischemic injury may be related to the change of eNOS subtypes.
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