中文摘要：

旨在通过研究胰岛素样生长因子1（IGF-1）对羊驼皮肤成纤维细胞的作用来了解IGF-1对羊驼毛皮生长发育的调控作用。本研究中，（1）利用免疫组织和细胞化学技术，研究IGF-1及其受体IGF-1R在成年羊驼皮肤和培养的成纤维细胞中的表达；（2）观察不同浓度体外重组人IGF-1对成纤维细胞增殖形态学的影响；（3）采用QRT-PCR技术检测添加不同浓度IGF-1对成纤维细胞的IGF-1R、转化生长因子β1（TGF-β1）和角质细胞生长因子（KGF）基因表达量的影响。结果：（1）IGF-1及其受体IGF-1R蛋白在成年羊驼皮肤和培养的成纤维细胞中均有表达；（2）添加IGF-1对成纤维细胞的数量和形态都有明显促进作用，10ng·mL-1组的效果最显著；（3）IGF-1影响成纤维细胞的IGF-1R、TGF-βl和KFG基因的表达。与对照组相比，10ng·mL-1时KGF基因表达水平是3．332倍，IGF-1R基因表达水平是4．479倍。TGF-β1基因表达水平最低为0．405倍，1ng·mL-1时为0．813倍，100ng·mL-1时为0．434倍。结果表明，成纤维细胞既是IGF-1作用的靶器官，又是内分泌器官。IGF-1促进细胞增殖，同时提高其IGF-1R和KGF基因表达量，降低TGF-β1基因表达量，提示IGF-1可通过调节其受体和内分泌因子的表达量而调控羊驼毛的生长发育。

英文摘要：

This study aimed to investigate the regulation of IGF-1 on promoting hair growth and development by studying the effect of IGF-1 on alpaca skin fibroblasts. In this study. （1）Analy- zing the expression of IGF-1 and its receptor IGF-1R in the alpaca skin and cultured fibroblasts by using immunohistochemical and immunocytochemistry techniques. （2） Observing the effect of re- combinant human IGF-1 in vitro with different concentrations on the proliferation and morpholog- ical changes of cultured alpaca skin fibroblasts. （3） Detecting the expression quantity of IGF-1, TGF-β1 and KGF genes in fibroblasts cultured in different IGF-1 concentrations by using QRT- PCR technology. Result. （1）The result of IGF-1 and its receptor IGF-1R protein expression was posi- tive in the alpaca skin and cultured fibroblasts. （2） Adding IGF-1 had significant growth promoting effect on the quantity and morphology of fibroblasts, especially the addition of 10 ng · mL 1. （3）IGF-1 could affect the gene expression of IGF-1R, transforming growth factor 131 （TGF-β1） and kerati-nocyte IGF-1 growt at the IGF-1R times at indicate expr h factor（KGF） in alpaca skin fibroblasts. Compared with the control group, when concentration of 10 ng · mL-1 , the expression level of KGF was 3. 332 times and ession level was 4. 479 times. TGF-β1 gene expression level was the lowest, 0. 405 10 ng · mL-1 ,0. 813 times at 1 ng · mL-1 and 0. 434 times that alpaca skin fibroblasts are the target organ of IGF-1 IGF-1 can induce the pro IGF-1R and KGF genes, modulate the growth and and endocrine factors liferation of alpaca reducing the expr skin fibroblasts, while ession of TGF-βI gene, development of alpaca hair by regulating at 100 ng · mL-1. The results , as well as endocrinal organ. improving expression level of which indicate that IGF-1 can the expression of its receptor