中文摘要：

与共焦显微镜相比，双光子荧光显微镜（TPM）在生命科学研究中具有较大的优势．其表现在避免了生物样品的紫外光损伤、光漂白和焦平面外的荧光干扰等．检测光路中不采用小孔光栅，对成像多自发荧光和高散射样品也有独特的优势，因此被认为是生物成像领域革命性的发展．

英文摘要：

A new two-photon fluorescence DNA probe, BMVEC, with bigger σ×Ф 1437 GM） was synthesized. The absorption titration shows that there is an interaction between BMVEC and DNA because the probe can intercalate the grooves in the double helix of DNA, which enhances its two-photon fluorescent intensity. Meanwhile the double-staining results in cancer cells by means of TPM demonstrate the exclusive nucleic labe-ling ability of BMVEC, and the positions and amounts of nuclei labeled by BMVEC are wholly the identical as those by DAPI. Moreover, the incident power of BMVEC is lower than that of DAPI. The results indicate that BMVEC can act as the two-photon fluorescent probe of DNA.