中文摘要：

目的探讨滇重楼不同居群间、同一居群不同植株间甾体皂苷活性成分在根茎、茎、叶中的代谢规律。方法采用超高效液相色谱法（UPLC）分别测定滇重楼不同居群间、同一居群不同植株间根茎、茎、叶中甾体皂苷活性成分的含量，并建立其UPLC图谱，对滇重楼不同居群间、同一居群不同植株间甾体皂苷活性成分在根茎、茎、叶的代谢规律进行研究。结果滇重楼不同居群间根茎、茎、叶中次生代谢产物重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ及总皂苷含量（P〈0．01）具有极显著差异，UPLC图谱中色谱峰个数为叶〉根茎〉茎；滇重楼同一居群不同植株间根茎、茎、叶中次生代谢产物重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ及总皂苷含量（P〈0．05）差异较小，UPLC图谱中色谱峰1、2、4、5在根茎、茎、叶均存在，但叶比根茎、茎分别还多11、16个色谱峰。结论滇重楼不同居群间、同一居群不同植株的茎中主要活性成分含量较低、种类较少；滇重楼甾体皂苷活性成分在同一居群不同植株间根茎、茎、叶中的代谢具有较稳定的居群遗传能力，在不同居群间的代谢存在较大的差异；滇重楼茎、叶是否可以作为其根茎的替代品入药，仍需进一步做药理、毒理及临床实验。

英文摘要：

OBJECTIVE To discuss the metabolism rule of the active steroidal saponins in the roots, stems and leaves of Paris polyphylla var. yunnanensis Franch belonging to different populations and different plants in the same population. METHODS UPLC was used to determine the contents of the active ingredients including polyphyllin Ⅰ , polyphyllin Ⅱ , polyphyllin Ⅵ, and polyphyllin Ⅶ from the roots, stems, and leaves, the UPLC map was established, the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software （ Version 2004 A） developed by the Chinese Pharmacopoeia Committee was used to analyze the number of peaks in the UPLC map, and One-way ANOVA was used to analyze their metabolism rule. RESULTS There was significant difference in the contents of polyphyllin Ⅰ , polyphyllin Ⅱ , polyphyllin Ⅵ, polyphyllin Ⅶ, and their total contents in the roots, stems, and leaves among different populations （ P 〈 0. 01 ）. The number of peaks in the UPLC map ranked in the folloing order ： leaf 〉 root 〉 stem. The difference among different plants of the same Paris polyphylla var. yunnanensis Franeh population was smaller. No. 1, 2, 4, 5 peaks all existed in the UPLC maps of the roots, stems, and leaves, but there were 11 and 16 more peaks in the leaves than the roots and stems, respectively. CONCLUSION There are fewer kinds of main active ingredients with low contents in the stems of Paris polyphylla var. yunnanensis Franeh. The population genetic capabilities of the metabolism of the steroidal saponins active ingredients are stable in the roots, stems and leaves of different plants from the same population, but there are large differences between different populations. Further pharmacological, toxicological, and clinical studies need to be done to determine whether the leaves and stems could take the place of root as an alternative medicine.