中文摘要：

目的探讨可吸收明胶海绵的生物相容性，为其作为骨髓间充质干细胞（BMSCs）的有效载体进行眼内移植奠定基础。方法体外培养BMSCs，接种于可吸收明胶海绵，观察BMSCs的黏附、增生情况，采用乳鼠视网膜细胞条件分化液诱导，观察BMSCs的分化情况并行免疫组织化学鉴定。结果在乳鼠视网膜条件分化液的环境中，接种后72h，BMSCs伸出伪足黏附于可吸收明胶海绵表面。诱导后的细胞呈现神经元细胞的典型形态，免疫组织化学显示神经元特异性烯醇化酶（NSE）和巢蛋白（nestin）阳性。结论BMSCs能够在可吸收明胶海绵上较好地黏附、增生及分化，可吸收明胶海绵具有良好的生物相容性。

英文摘要：

Objective Bone mesenchymal stem cells （BMSCs） is a multiple potential stem cells and has a ability to differentiate into retinal cells. Therefore, BMSCs is becoming a seeding cells for the intraoeular transplantation. The aim of this study was to investigate the biocompatibility of absorbable gelatin sponge and the possibility of gelatin sponge as BMSCs scaffold in intraocular transplantation. Methods BMSCs from femur marrow of Wistar rat aged 8 days were cultured and amplicated in DMEM/F12 with 10% fetal bovine serum. The forth passage of cells were collected and identified by the detect of surface antigen of the cells using flow cytometry. The cells were co-cultured with absorbable gelatin sponge. The adhesive and proliferative biological activity of BMSCs on absorbable gelatin sponge was observed under the light microscope and scanning electron microscope. After 7-day cultivation,BMSCs were induced by conditioned medium of neonate rat retinal cells. The morphological changes of induced BMSCs on absorbable gelatin sponge were observed under the scanning electron microscope. The expression of neuron specific enolase（NSE） and nestin of induced cells was detected by immunohistochemistry. Results The flow cytometry showed that cultured cells were positive response for CD71 and CD44 and negative response for CD45 and CD34. After co-cultured with absorbable gelatin sponge under the condition of retinal cell-conditioned medium, BMSCs sticked to gelatin sponge in 24 hours and stretched out many pseudopods linked with the gelatin surface in 72 hours. Seven days later,gelatin sponge degraded and the survival cells could been seen under the scanning electron microscope. The cells were crosslinked and secreted large quantity of extra-cell matrix surrounding the gelatin sponge. BMSCs presented classical neuronal modality and connected with each others after induced by conditioned medium of neonate rat retinal cells. Immunohistochemistry showed that induced BMSCs had the positive response for NSE and nestin, showing