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艾丁湖盐碱环境分离菌株特异性多重PCR快速鉴定
  • ISSN号：0258-7971
  • 期刊名称：《云南大学学报：自然科学版》
  • 时间：0
  • 分类：Q93-3[生物学—微生物学]
  • 作者机构：[1]云南大学云南省微生物研究所
  • 相关基金：国家自然科学基金(30600001);教育部新世纪优秀人才支持计划;云南省中青年学术和技术带头人后备人才基金;云南省自然科学基金(2004 C0002Q)
作者： 吴晋元[1], 职晓阳[1], 唐蜀昆[1], 李文均[1]
关键词： 糖单孢菌, 拟诺卡氏菌, 特异性PCR, 多重PCR
中文摘要：

参照糖单孢菌属(Saccharomonospora)和拟诺卡氏菌属(Nocardiopsis)的特异性引物,建立多重PCR反应体系快速鉴定分离自新疆艾丁湖盐碱环境分离的118株嗜盐菌株,检测出14株糖单孢菌属菌株,17株拟诺卡氏菌属菌株.研究发现2株普氏菌属菌株出现针对糖单孢菌属的特异性条带,退火温度提高至61℃时可消除这种假阳性扩增.应用多重PCR快速鉴定方法可提高对盐碱环境中的这2个类群初步鉴定以及筛选工作的速度和效率.

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期刊信息
  • 《云南大学学报：自然科学版》
  • 中国科技核心期刊
  • 主管单位:云南省教育厅
  • 主办单位:云南大学
  • 主编：张力
  • 地址：昆明市呈贡新区
  • 邮编：650500
  • 邮箱：yndxxb@ynu.edu.cn
  • 电话：0871-5033829 5031498 5031662
  • 国际标准刊号：ISSN：0258-7971
  • 国内统一刊号：ISSN：53-1045/N
  • 邮发代号:64-29
  • 获奖情况:
  • 1999年荣获全国优秀高校自然科学学报及教育部优秀...,1997年荣获全国第二届优秀科技期刊评比二等奖,1995年全国重点大学优秀科技期刊评比二等奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘（网络版）,美国数学评论（网络版）,英国农业与生物科学研究中心文摘,波兰哥白尼索引,德国数学文摘,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）
  • 被引量:11696