中文摘要：

制备出3种不同结合比的酶标抗体，对检测条件进行了优化，建立了标记抗体固相抗原直接竞争ELISA（cdELISA）检测丁草胺的方法。结果表明，酶标抗体克分子比为1．55时偶合物活性最高，包被浓度1μg·mL^-1，酶标抗体800倍稀释，稀释液采用2×PBS，不含BSA、甲醇，建立的标准曲线IC50=1．7ng·mL^-1，检测限为0．006ng·mL^-1，检测范围0．06-2616．7ng·mL^-1，批内变系数9．25％，批间变异系数23．55％。蒸馏水以及稀释10倍后的矿泉水、池塘水、稻田水添加1ng·mL^-1和10ng·mL^-1的丁草胺，用所建立的方法检测。平均回收率分别为93．27％、158．50％、119．75％、124．51％。本研究为进一步开发丁草胺免疫分析试剂盒奠定了基础。

英文摘要：

A competitive enzyme-linkod immunosobent assay（ELISA） for the detection of the herbicide butachlor was development, which based on enzyme-labeled antibody and solid antigen （cdELISA）. Three molar ratio of enzyme-labeled antibodies were prepared, the one with 1.55 of molar ratio had higher activity, the optimized cdELISA performed with coating antigen of 1 μg·mL^-1 and enzyme conjugate of 800- fold dilution in 2×PBS dilution solution without protecting agent BSA and solvent methanol, has a butachlor IC50 of 1.7 ng·mL^-1 and detection limit of 0.006 ng·mL^-1, the detection range was 0.06-2 616.7 ng·mL^-1,the inter CV was 9.25% and intra CV was 23.55%. The water samples after 10-fold dilution, fortified with butachlor, were analyzed according to this mothod. The average recovery rate for mineral water, pond water, rice filed water and distilled water was 158.50%, 119.75%, 124.51%,93.27%, respectively, which proved the suitability of the method to develop commercial immunoassay kit for butachlor.