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中文摘要：
[目的]考察了4种分离提取方法对生物转化液中转化产物11-α-羟基-坎利酮提取率的影响。[方法]4种分离提取方法分刖为文献法、浸泡法、洗脱法和纠浆法。[结果]11-α-羟基-坎利酮主要游离于转化液或附着于茼丝体外表面，洗脱法和浸泡法埘11-α-羟基-坎利酮具钉较好的分离效果。采用400ml乙峻乙酯洗脱，11-α-羟基-坎利酮提取率为96．0％；经乙酸乙酯浸泡90min，11-α-羟基一坎利酬提取牢达到98．8％。[结论]该方法简单高效，具有工业化应用潜力。
英文摘要：
[Objective] The aim was to investigate the effect of four extraction meth- ods on extraction ratio of 11-α-Hydroxy-Canerenone from biotransformation broth. [Method] Previous reported method, soaking, elution method and homogenate meth- ods were used. [Result] Results showed that more than 90% of the biotransformed 11-α-Hydroxy-Canerenone existed in the broth supernatant and mycelia surface. Elu- tion and soaking methods were effective for 11-α-Hydroxy-Canerenone extraction. Extraction yields of 96.0% and 98.8% were obtained by eluting with 400 ml of ethy- lacetate and soaking with ethylacetate for 90 min, respectively. [Conelusion] The method is simple and efficient, showing industrial application potential.
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