中文摘要：

目的：建立一种快速、简便、高效的鸡胚整胚原位杂交地高辛标记的RNA探针的制备方法。方法：应用RT-PCR方法从鸡胚总RNA中扩增目的片段，连接到PGM-T克隆载体上，分别利用PGM-T载体中的SP6和T7 RNA 聚合酶上的启动子，以线性化的DNA为模板体外转录成地高辛标记的正反义 RNA探针。结果：成功得到地高辛标记的 RNA 探针，探针在鸡整胚原位杂交中有杂交信号。结论：成功转录得到地高辛标记的正反义探针，为后续的以鸡胚为模型整胚原位杂交试验做好了探针的准备。

英文摘要：

Objective：To develop a fast,simple and efficient protocol for whole-mount in situ hybridization of chick embryos with digoxigenin（DIG）-Labeled RNA probe.Methods：The target fragment of gene was ob-tained by RT-PCR through total RNA of chicken,using the RNA polymerase promoter,based on linear DNA template in vitro transcribed into DIG-Labeled RNA probe.Results：Obtained the DIG-Labeled RNA probe and positive signals were viewed in chick embryos.Conclusion：The successful preparation of DIG-La-beled RNA probes lays an experimental foundation for whole-mount in situ hybridizatation of chick embry-os which be used as an animal mode.