中文摘要：

喜盐鸢尾（Iris halophila Pall.）及其变种蓝花喜盐鸢尾（I.halophila Pall.var.sogdiana（Bung）Grubov）因耐盐碱及其多种花色而具有盐碱地园艺开发价值。本文根据喜盐鸢尾内轮花被转录组测序结果,利用基因特异性引物从这2种植物中分别克隆了编码查尔酮合成酶（CHS）、查尔酮异构酶（CHI）、类黄酮-3＇,5＇-羟基化酶类（F3＇5＇H-like）等基因的部分片段,并对它们在内轮花被中的表达水平进行实时定量PCR分析。序列分析结果确认在喜盐鸢尾中所克隆的CHS（311 bp）、CHI（457 bp）、F3＇5＇H-like（496 bp）3个基因（部分）未见文献报道与NCBI等数据库记录。其中F3＇5＇H-like基因与经典的属于细胞色素P450CYP75A亚家族的F3＇5＇H不同,而与万带兰的F3＇5＇H-like同属于CYP76AB亚家族,为一类新的蓝花相关基因。实时定量PCR表达分析结果表明,与黄花的喜盐鸢尾相比,蓝花喜盐鸢尾中CHS与F3＇5＇H-like显著上调表达,可能是其花色不同于喜盐鸢尾的主要原因。

英文摘要：

Iris halophila Pall. and its variety I. halophila Pall. var. sogdiana （Bung）Grubov are potential hor- ticultural plants for arid and saline-alkali land because of their drought and salt tolerant characteristics and various flower color. In order to elucidate the mechanism of different pigmentation in these two species, we cloned 3 partial segments of key genes relating to anthocyanin biosynthesis, named chalcone synthase （ CHS）, chalcone isomerase （CHI） and flavonoid-3＇, 5＇-hydroxylase like （F3＇5＇H-like）, from perianth of young flowers of these two Iris plants. Retro-transcription PCR method were employed in cloning and gene-specific primers were designed based on a transcriptome sequencing. The expression of these 3 genes were characterized by a real-time PCR assay. Se- quence analysis showed that these 3 genes were first reported in I. halophila Pall.. Among these genes, F3＇5＇H- like was different from the classical F3＇5＇H which belonged to CYP75A subfamily of cytochrome P450 superfami- ly,it was a homolog of Vanda coerulea Griff. ex Lindl. F3＇5＇ H-like gene which belonged to a new subfamily CYP76AB, a novel type of blue flower related genes. Expression analysis indicated that compared with in the yellow flowered I. halophila Pall. , CHS and F3＇5＇H-like were up-regulated in I. halophila Pall. var. sogdiana （Bung） Grubov and responsible for its blue-violet flowers.