中文摘要：

使用质粒DNA评价法和ICP-MS研究贵州织金地区海陆过渡相的晚二叠世煤及大同地区陆相的侏罗纪煤在实验燃烧炉中燃烧排放PM10对质粒DNA的氧化性损伤能力及其与重金属元素组成的关系。选择2种煤的蜂窝型煤进行燃烧实验，以木炭作为对照组进行研究。对比各种燃料燃烧产生的PM10的DNA损伤实验结果发现：全样和水溶性组分所产生的DNA损伤率相似，说明水溶性组分是造成氧化性损伤的主要来源；海陆过渡相的织金煤燃烧排放PM10的氧化性损伤能力明显比陆相大同煤燃烧产生的PM10的高，2种煤的燃烧排放PMlo对DNA损伤能力均比木炭本底的高。对3种PM10中的水溶性微量元素进行分析，水溶性的As，Cd，Cu，Ge，Hg，Mo，Pb，Sb，Se，v和Zn在海陆过渡相的织金煤燃烧排放的PM10中含量较高，在陆相的大同煤燃烧排放的PMlo中较低，在木炭本底燃烧排放的PM10中最低。PM10中较高的水溶性Zn和Pb含量，说明环境大气PMlo中的Zn和Pb可能与燃煤排放相关，而Hg元素因为较强的挥发性而在PM10中未得到富集。水溶性的微量元素总量及大部分水溶性的单个微量元素均表现出与颗粒物对DNA损伤率一致的变化规律，说明燃煤产生的PMl0与其中的水溶性组分密切相关。

英文摘要：

Plasmid DNA assay and inductively coupled plasma mass spectrograph （ICP-MS） were applied to study the relationship between oxidative capacity based on the DNA damage and the heavy metal compositions of the inhalable particles generated by coal burning. The coals selected for experiments include a Late Permian coal in Zhijin area which was formed in a transitional marine-continental environment and a Jurassic coal in Datong area which was formed in a , ontmental fluvial and lacustrine environment. The honeycomb briquette coals and a wood charcoal sample were burnt in the pre-conditioned stove to generate inhalable particles （PM10, airborne particles with aerodynamic diameters less than 10 ram）. Three PM10 samples were collected for the plasmid DNA assay and ICP-MS analyses. The results show that the whole PM10 sample solutions and their soluble fractions have produced a similar level of DNA damage rates for all three samples, indicating that the DNA damage of PM10 is mainly sourced from their water-soluble fractions. At the same dosage, the PMl0 generated by burning the transitional facies Zhijin coal has a higher DNA damage rate than that by burning the continental facies Datong coal. The oxidative damage of the PM10 emitted by coal burning is higher than that by wood burning. Total analyzed water-soluble heavy metals as As, Cd, Cu, Ge, Hg, Mo, Pb, Sb, Se, V and Zn in PM10 generated by burning Zhijin coal have higher concentrations than that in the PM10 generated by burning Datong coal, and the contents of these elements in the PM10 emitted by coal burning are obviously higher than that by wood burning. The PM10 emitted by coal burning in this experiment is obviously enriched with water-soluble Zn and Pb, implying that the Zn and Pb in the ambient PM10 may come from coal burning emission. The variation trend of the these water-soluble heavy metals in the PM~0 samples is the same as the DNA damage rates, demonstrating that the oxidative capacity of coal burning PM10 is mainly from these water-solu