中文摘要：

目的研究mi R-21在人脑胶质瘤细胞中调控FASLG基因表达的作用效果及分子机制。方法应用实时定量PCR方法检测人脑胶质瘤及其癌旁组织中mi R-21及FASLG基因的表达。转染mi R-21的前体（mi R-21precursor）至人脑胶质瘤U87MG细胞,然后应用Western blot法检测FASLG蛋白的表达。应用荧光素酶报告基因表达分析实验分析mi R-21能否与FASLG基因的3＇非翻译区（3＇UTR）特异性结合。结果人脑胶质瘤组织中的mi R-21m RNA的表达水平明显比癌旁组织少,而FASLG m RNA则增加显著,二者的表达水平呈明显的负性相关。转染mi R-21 precursor能够显著上调胶质瘤U87MG细胞中mi R-21的表达,而在mi R-21高表达的胶质瘤U87MG细胞中,FASLG的蛋白表达下调显著。mi R-21能够特异性地与FASLG基因的3＇UTR中的种子区结合,负性调节荧光素酶报告基因的表达。结论 mi R-21与FASLG基因与人脑胶质瘤的发生相关,mi R-21在胶质瘤细胞中能够靶向沉默FASLG基因。

英文摘要：

Objective To investigate the regulation effect and molecular mechanism of miR-21 on FASLG gene in human brain glioma. Methods The expression of miR-21 and FASLG mRNA in human brain glioma tissues and homologous peri-cancerous tissues was detected by fluorescence quantitive PCR. The miR-21 precursor was transfected into human brain glioma U87MG ceils, and then the expression of FASLG protein was determined by Western blot. The direct connection between miR-21 and 3＇ UTR of FASLG gene was proved with the luciferase reporter gene expression analysis essay. Results The expression level of miR-21 mRNA increased markedly in human brain glioma tissue than in homologous peri- cancerous tissue, but decreased for FASLG mRNA, both showing a negative correlation. The transfection with miR-21 precursor into U87MG cells could up-regulate the expression level of miR-21. The expression of FASLG protein was downregulated remarkably in U87MG cells with miR-21 over-expression. The direct connection between miR-21 and 3＇ UTR of FASLG gene was determined with the luciferase reporter gene expression analysis essay, and miR-21 could negatively regulate the luciferase activity. Conclusion Both of miR-21 and FASLG gene were involved in the genesis of human brain glioma, miR-21 could silence the FASLG gene expression targetly in human brain glioma cells.