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中文摘要：
采用Hypersil ODS2（4.6 mm×250 mm,5 μm）色谱柱,以磷酸盐缓冲溶液（pH=2.6）为流动相,流速0.8 mL/min,检测波长210 nm,建立了山楂中柠檬酸、苹果酸等有机酸的高效液相色谱分析方法.其中柠檬酸和苹果酸的线性范围分别为0.50～50.04 μg、0.10～10.00 μg;3个浓度水平的回收率分别为95.5%～96.6%、100.2%～103.7%,RSD分别为0.11%～0.38%、0.41%～2.53%;检出限分别为0.04 μg、0.03 μg（S/N=3）.测定了5种不同产地山楂果肉及山楂核中的柠檬酸、苹果酸的含量.所建立的方法快速﹑灵敏度高、重复性好,可用于山楂中有机酸的测定.
英文摘要：
Hawthorn is a traditional Chinese medicine and fruit. It contains numerous organic acids, it is reported that organic acids have the ability to cure indigestion and have many other functions. A quantitative reversed-phase high performance liquid chromatography method using diode array detector has been developed for the determination of citric acid and malic acid in hawthorn. A Hypersil ODS2 cohumn was used with phosphate buffer solution （pH =2.6） as the mobile phase at a flow rate of 0.8 mL/min. The cohumn temperature was 25℃ and the UV detection wavelength was 210 run. The calibration curve for citric acid and malic acid was in good linearity in the range of 0.50 - 50.04μg with the regression equation Y = 78. 2× - 0.08, r=0.9999 and0.10-10.00 μg, Y=65. 1X-0.065,r =0.9999, respectively. The average recoveries of three concentration leveds for citric acid and malic acid in hawthorn were 95.5% - 96.6% and 100. 2% - 103.7%, respectively. RSD were 0.11% -0.38% and 0.41% -2.53%, respectively. The detection limits for citric acid and malic acid were 0.04μg and 0.03 μg （ S/N = 3）, respectively. The results indicate that this method is fast, sensitive, reproducible and practical for the routine analysis of organic acids in hawdorn;
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