中文摘要：

目的探讨碱性成纤维细胞生长因子（bFGF）对动脉粥样硬化（AS）模型大鼠脑顶叶皮质与大脑中动脉M1段血管结构与功能的影响。方法随机将30只雄性Wistar大鼠分为正常对照组、AS组、bFGF治疗组。除正常对照组外在喂食开始时一次性腹腔注射维生素D3（6×104IU/kg）加高脂配方饲料喂养,6周后bFGF治疗组腹腔注射bFGF 9.5μg/kg,每日2次,连续14 d。8周后处死大鼠,测各组血脂、脑组织生化指标、大脑中动脉壁胆固醇含量及Caspase-3蛋白表达水平;观察大脑中动脉M1段、脑顶叶皮质的病理改变。结果高脂饲料饲养6周后早期动脉粥样硬化形成,bFGF能降低血脂、血管壁胆固醇含量及Caspase-3蛋白表达水平（P〈0.05）,降低脑组织丙二醛（MDA）含量,改善脑组织超氧化物歧化酶（SOD）和一氧化氮（NO）活性（P〈0.05）;减轻AS所致大脑中动脉M1段与脑顶叶皮质的病理性损害（P〈0.05）。结论 bFGF能改善AS模型大鼠的血脂与氧化能力、减轻脑组织与血管壁的病理损害,这些变化提示bFGF具有保护脑组织与脑血管的作用。

英文摘要：

Objective To study the protective effects of basic fibroblast growth factor（bFGF） on cortex of parietal lobe and M1 segment of middle cerebral artery in atherosclerosis（AS） model rats.Methods A total of thirty male adult Wister rats were randomly divided into the normal control group,the AS model group and the bFGF treatment group.Except for the control group,rats of the other two groups were injected with a single dose of vitamin D3（6×104IU/kg） and loaded with high fat diet for six weeks continuously.The bFGF was injection into the abdominal cavity after six weeks in the bFGF treatment group at 9.5 μg/kg for two weeks continuously,and an identical volume saline was given to the AS model group and the normal control group.After eight weeks,all the rats were sacrificed.Then,the serum total cholesterol,triglyceride,low density lipoprotein and high density lipoprotein were respectively assayed,and the colorimetric method was used to detect the level of superoxide dismutase（SOD）,Nitric-Oxide（NO） and the content of Malondialdehyde（MAD） in the brain tissues.The cholesterol and Caspase-3 content of the M1 segment of middle cerebral artery were respectively detected by the high-performance liquid chromatography and Western blot.As well the pathological lesion of the M1 segment of middle cerebral artery and cortex of parietal lobe were observed under a light microscopy and quantitative analysis was performed by cell morphometric technique.Results The early AS plaques were presented after six weeks by hyper lipid foods.Compared with those of the AS model group,the level of serum lipid,the content of cholesterol and Caspase-3 expression in the bFGF treatment group obviously decreased（P0.05）;The content of MAD and activity of SOD and NO also obviously decreased in the brain tissues（P0.05）;The pathologic injury of both the brain tissue and the M1 segment of middle cerebral artery decreased（P0.05）.Conclusion bFGF may play an important role in antagonizing the brain artery and cortex injuri