中文摘要：

目的：探讨寻常型银屑病患者血清中白细胞介素17（IL-17）的表达水平及IL-17刺激后HaCaT细胞分泌白细胞介素6（IL-6）和白细胞介素23（IL-23）的变化,阐明其临床意义及紫草素的干预作用。方法：以25名正常对照者、29例寻常型银屑病患者和不同组别HaCaT细胞（空白对照组、IL-17刺激24h组、IL-17刺激36h组、IL-17刺激48h组、紫草素＋IL-17组、环孢素A＋IL-17组和IL-17组）为研究对象,采用双抗体夹心酶联免疫吸附法（ELISA）检测寻常型银屑病患者血清IL-17水平和HaCaT细胞各组上清液中IL-6、IL-23水平;实时荧光免疫定量-聚合酶链式反应法（RT-PCR）检测各组HaCaT细胞中IL-6和IL-23p19mRNA表达水平;采用细胞计数盒-8（CCK-8）法检测各组HaCaT细胞活力。结果：与正常对照组比较,银屑病组患者血清IL-17水平明显升高,以重度皮损银屑病组升高最为明显（P〈0.05）;IL-17刺激24、36和48h组HaCaT细胞及其培养上清中IL-6和IL-23水平及其mRNA表达水平均高于空白对照组（P〈0.01）;紫草素＋IL-17组和环孢素A＋IL-17组HaCaT细胞及其培养上清中IL-6和IL-23水平及其mRAN表达水平均低于IL-17组（P〈0.05）。与空白对照组比较,其他各组细胞活力差异无统计学意义（P〉0.05）。结论：银屑病患者血清IL-17表达水平升高,尤其是重度皮损银屑病患者血清IL-17表达水平升高明显,IL-17可促进HaCaT细胞分泌IL-6和IL-23,呈时间依赖性,紫草素可抑制IL-17的促炎症作用。

英文摘要：

Objective：To explore the expression level of serum interleukin-17（IL-17） in the patients with psoriasis vulgaris and the changes of interleukin-6（IL-6） and interleukin-23（IL-23） levels secrered by the HaCaT cells after stimulated with IL-17,and to clarify its clinical significance and the intervention effect of shikonin.Methods：Twenty-five normal controls,29 patients with psoriasis vulgaris and different groups of HaCaT cells（blank control group,IL-17-24 hgroup,IL-17-36 hgroup,IL-17-48 hgroup,shikonin＋IL-17 group,CsA＋IL-17 group and IL-17 group）were used as the subjects.The levels of serum IL-17 in the patients with psoriasis vulgaris and the levels of IL-6 and IL-23 in supernatant of HaCaT cells in various groups were measured by double antibody sandwich enzyme-linked immunosorbent assay（ELISA）and real-time polymerase chain reaction RT-PCR was used to detect the expression levels of IL-6and IL-23p19 mRNA in HaCaT cells in various groups.At the same time,Cell Counting Kit-8（CCK-8）method was used to detect the viabilities of HaCaT cells in various groups.Results：The level of serum IL-17 of the patients in psoriasis vulgaris group was increased compared with nomral control group,especially in the severe skin lesion group（P〈0.05）.The levels of IL-6and IL-23 in HaCaT cells and supernatant and their mRNA expression levels in IL-17-24 h,IL-17-36 hand IL-17-48 hgroups were significantly higher than those in blank control group（P〈0.01）.The expression levels of IL-6 and IL-23 in HaCaT cells and supernatant and their mRNA expression levels in shikonin＋IL-17 and CsA＋IL-17 groups were lower than those in IL-17 groups（P〈0.05）.No statistical differences were found in the cell viabilities between each drug treatment group and blank control group（P〉0.05）.Conclusion：The expression level of IL-17 in the patients with psoriasis vulgaris is significantly increased,especially in the patients with severe psoriasis vulgaris.IL-17 can promote the secretion of IL-6 and IL-23