在温室中用PEG模拟干旱胁迫,比较了内生真菌感染(EI)和非感染(EF)的高羊茅(Festuca arundinacea Schreb.cv.Millennium)种群的几项与水分生理相关的指标.结果表明在对照和轻度胁迫时,EI植株的分蘖、叶长、渗透势、游离脯氨酸含量及生物量与EF种群相比都没有显著差异;重度胁迫条件下,复水后EI植株叶片展开程度明显大于EF植株,根系活力显著高于EF植株(P<0.05),说明重度干旱胁迫下内生真菌感染对高羊茅的恢复生长有一定的增益作用.
Endophytes have been defined as fungi that live for a significant part of their life cycle internally and asmptomatically in plants. Endophytes and their host grasses are mutually symbiotic. In the past, much work has been done on the beneficial effect of endohyte infection on biotic and abiotic stresses resistance of grasses, especially the biotic stress resistance. In this paper Festuca arundinacea cv Millennium infected by Neotyphodium coenophialum was chosen as an experimental materials. The experiment was carried out in the green house and osmotic potential, number of tillers, cumulative length of leaves, free proline content, root activity, total biomass of endophyte-infected (EI) and endophyte-free(EF) populations of tall rescue under normal and osmotic stress conditions were compared. The results indicated that: osmotic potential, number of tillers, leaf length, biomass, free proline content and root activity of EI population were close to those of EF population under control and mild stress ; EI and EF populations had little damage under mild stress, which indicated that Millennium possessed a certain extent drought of resistance. When a more severe osmotic stress was imposed, EI population had higher root activity, especially after recovering began and the difference was significant. At the same time , leaves of EI population extended wider and longer, when they were released from the stress. Thus we concluded that Neotyphodium infection appear to be beneficial to host performance after the severe stress was released. In tother words, endophyte enhances recovery ability of the host. The accumulation of free proline is sensitive to the stress intensity and stress time.