中文摘要：

以壳聚糖微球为载体，采用吸附-交联法固定化β-葡萄糖苷酶。采用正交试验设计确定最佳固定化条件。β-葡萄糖苷酶的最佳固定化条件为：pH值5．0，酶用量48．6IU／g（以绝干壳聚糖计，下同）的β-葡萄糖苷酶经壳聚糖微球吸附12h后，在25℃、1．0％的戊二醛存在下交联2h，得到酶活力为41．76IU／g和酶活回收率为85．93％的固定化酶。

英文摘要：

β-Glucosidase was immobilized on chitosan beads by adsorption and crosslinking. The method of orthogonal test was used to investigate the optimal condition of immobilization. The optimal condition of immobilization was as follows：β-glucosidase dosage 48.6 IU/g dry chitosan was absorbed with chitosan beads at pH value 5.0 for 12 h, followed by crosslinking at 25 ℃ for 2 h in 1.0 % glutaraldehyde（GA） solution. The activity of immobilized β-glucosidase and recovery rate of β-glucosidase activity were 41.76 IU/g （ based on dry chitosan） and 85.93 %, respectively.