中文摘要：

为研究低温胁迫下抗冻基因ld4对细胞应激颗粒（Stress granules）形成的影响,从南极鱼Lycodichthys dearborn的多聚AFPⅢ基因ld12 c DNA中克隆得到AFPⅢ蛋白四聚体ld4,将其转染到He La细胞中,并采用反转录PCR（RT-PCR）、Western blot方法检测到目的基因ld4在细胞中成功表达,进而采用细胞免疫荧光方法检测细胞在低温（10℃）胁迫下细胞应激颗粒的形成情况。结果表明,与对照组相比较,在低温胁迫下,表达ld4基因的He La细胞中应激颗粒明显减少。研究表明,ld4基因的表达在低温胁迫下能显著降低He La细胞中应激颗粒的形成,试验结果可为ld4基因作为鱼类耐寒育种的候选基因提供理论依据。

英文摘要：

An AFPⅢ tetramer gene ld4 from Antarctic eelpout Lycodichthys dearborni was transfected into He La cell（ human cervical cancer cell） to investigate the relationship between antifreeze gene ld4 and cell stress granule formation in response to cold stress. Reverse Transcription PCR（ RT-PCR） and Western blot were applied to verify the ld4 gene expression in mRNA and protein levels in ld4 transfected He La cell. Further,cellular stress granules formation was detected under cold stress（ 10 ℃） and compared with that in the control cell by immunofluorescence. There was significant decrease in the stress granules formation in ld4 transfected He La cell under the cold stress than in the control,indicating that ld4 may enhance cellular cold resistance by reducing the formation of cellular stress granules. The findings provide a mechanism for ld4 gene as a candidate gene in breeding of cold resistant fishes.