中文摘要：

目的通过对比不同纳米颗粒钛膜表面成骨细胞的合成能力，评价其生物相容性。方法采用直流磁控溅射法通过控制温度（常温、100℃、250℃、380℃）构建4级纳米颗粒钛膜，将SD乳鼠第3代成骨细胞接种于其表面及未镀膜的钛片表面，对表面成骨细胞上清液中骨钙素（OC）含量进行检测。结果在7、14d时，随着时间的延长，各实验组和对照组样本表面的细胞上清液中OC含量均增大。在7d时，对照组与其他各组相比，OC含量间差异均有统计学意义（P〈0．05）。在14d时，100℃实验组表面OC含量增幅最大，100℃实验组与380℃实验组、对照组、空白组相比，其差异具有统计学意义（P〈0．05），250℃实验组与380℃实验组、对照组、空白组相比，其差异也具有统计学意义（P〈0．05）。结论钛表面纳米改性有利于其生物相容性，不同纳米粒径可以影响成骨细胞的合成功能。

英文摘要：

Objective To compare the synthetic ability of osteoblasts on the surface of different nano-granule titanium films and investigate the correlation between nanophase titanium films and cellular biocompatibility. Methods Four different nano-granule titanium films were produced by direct current magnetron sputtering, at ambient, 100 ℃, 250 ℃, 380 ℃ substrate temperature, respectively. Rat osteoblasts were seeded on the surface of four treated groups of titanium film samples and non-treated Ti sample （control group）. The production of osteocalcin （OC） in all five groups were detected by using double antibody sandwich enzyme-linked immunosorbent assay. Results The production of OC increased gradually from day 7 to day 14 in all groups. In the control group, it showed significant differences with other five groups on day 7. On day 14, the production of OC in 100 ℃ group was the highest, and it showed significant differences with 380 ℃, control group and blank group. In 250 ℃ group, the production of OC also showed significant differences with 380 ℃, control group and blank group（P〈0.05）. Conclusion Titanium with nano-modified surface had good biocompatibility and different nano-granule titanium films could affect the synthesis of osteoblasts.