中文摘要：

目的探索金属硫蛋白Ⅰ/Ⅱ（MetallothioneinⅠ/Ⅱ,MTⅠ/Ⅱ）对丙烯酰胺诱导星形胶质细胞损伤的保护作用。方法采用新生C57野生型（MT＋/＋）和MT敲除型（MT-/-）小鼠进行大脑皮层星形胶质细胞原代培养,并采用免疫细胞化学法鉴定细胞纯度。MT＋/＋和MT-/-两种细胞分别给予0.01、0.1和1 mmol/L丙烯酰胺处理,于不同时间点检测其细胞存活率,乳酸脱氢酶（LDH）漏出率和细胞凋亡率。结果细胞培养至15 d,两种细胞在形态学上无明显差异,细胞纯度达到90%以上。丙烯酰胺低剂量组（0.01 mmol/L）作用24 h后,MT-/-细胞存活率明显低于MT＋/＋,高剂量组（1 mmol/L）作用时间短于24 h,可见MT＋/＋细胞的存活率明显高于MT-/-,随着作用时间延长后,未见明显差异。丙烯酰胺作用24 h后,LDH漏出率和细胞凋亡率均随剂量依赖性增加,且丙烯酰胺中低剂量组（0.01和0.1 mmol/L）MT-/-细胞LDH漏出率和凋亡率明显高于MT＋/＋细胞。结论 MTⅠ/ⅡI对丙烯酰胺引起的星形胶质细胞损伤具有一定的保护作用。

英文摘要：

Objective To investigate the effects of metallothioneinⅠ/Ⅱ in acrylamide-induced injury in astrocytes.Methods Primary cultured astrocytes were obtained from cerebral cortex of 1 to 3 d old MT＋/＋（wild type） and MT-/-（MT-knock out type） mice.The cells purity was identified by immunocytochemical method.MT＋/＋and MT-/-astrocytes were exposed to acrylamide（0.01,0.1 and 1mmol/L） for various periods.Cell viability,LDH leakage and apoptosis rate were evaluated.Results No obvious differences were found in the morphorlogy of MT＋/＋and MT-/-up to 15 days culture in vitro,and the cell purity was 90% as determined by immunocytochemical method.Acrylamide treatment decreased the cell viability by concentration-dependent manner in both MT＋/＋and MT-/-astrocytes.The cell viability of MT＋/＋astrocytes was higher than that of MT-/-astrocytes after treated with acrylamide at 0.01 mmol/L for 24 h.In the cells treated with acrylamide at 1 mmol/L,MT＋/＋astrocytes showed higher viability than MT-/-cells after 6 - 24 h exposure,but no significant difference was found between MT＋/＋and MT-/-cells as the exposure periods were extended.Both the LDH leakage and apoptosis rate in MT-/-astrocytes were higher than that in MT＋/＋cells following treatment with acrylamide at 0.01 and 0.1 mmol/L.Conclusion These results indicate that metallothioneinⅠ/Ⅱ can protect astrocytes against acrylamide-induced injury.