中文摘要：

对杨树与落叶松-杨栅锈菌互作中活性氧（ROS）产生、抗性相关酶活性动态变化与寄主抗病性的关系进行研究。结果表明,不同杨树种或品种与该锈菌不同小种菌系的互作进程中寄主叶片ROS代谢和抗性相关酶活性变化存在明显差异。免疫、抗病组合寄主超氧阴离子（O2·-）产生速率和过氧化氢（H2O2）含量分别于接种后0.5,1天时迅速爆发且升幅最大,此后继续出现1～2个高峰,整个进程中明显高于感病组合;超氧化物歧化酶（SOD）、过氧化物酶（POD）活性与杨树品种抗病性呈负相关;免疫、高抗组合杨树在接种2天时苯丙氨酸解氨酶（PAL）活性快速升高且强度大,而感病组合无明显变化;几丁质酶活性与寄主抗病性呈正相关。

英文摘要：

The relationship between the host resistance and dynamic changes of superoxide anion （ O 2·- ）,hydrogen peroxide （H2O2）,peroxidase （POD）,phenylalanine ammonia lyase （PAL） and chitinase was analyzed in the interaction between poplar species or varieties （Populus deltoides,P. szechuanica,Populus × euramericana cv.‘Guarieto’,Populus × euramericana cv. ‘Polska 15 A’,Populus deltoides × P. nigra） and Melampsora larici-populina （ MLP） isolates （Gl052,Sb052）. The results showed that the host ROS metabolism and the changes of the resistance-related enzymes were significantly different in the interaction between different poplars and different MLP isolates. The rate of O 2·- production and content of H2O2 in the host of immune,resistance species and varieties quickly increased on the highest level respectively at 0. 5 day and 1day after inoculation,and appeared 1 ～ 2 peaks thereafter,but the ROS of the susceptible varieties lightly increased through the process. The activities of SOD and POD were negatively correlated with the poplar resistance. PAL activity increased rapidly and infensively in 2 day in the host of immune and high resistance poplars,while it did not obviously change in the susceptible one after inoculation. The chitinase activity was positively correlated with poplar resistance.