中文摘要：

目的初步探讨广谱趋化因子抑制先导物P1肽的活性及功能,为过度炎症反应的负调控提供理论基础。方法 MTT法检测P1肽的细胞毒性后,选择合适的浓度,应用液相芯片技术、钙流实验、RT-PCR和ELISA等方法对P1肽的活性和作用特点进行分析。结果 CXC类的IL-8、IP-10以及CC类的MCP-1、MIP-1β炎症趋化因子的产生量在P1肽的作用下有明显下降,且这两类趋化因子mRNA的表达量均有明显下降,进一步的实验显示P1肽不通过TTP途径降解mRNA;同时较之LPS炎症模型组,P1肽作用下的细胞炎症因子TNF-α的分泌水平有明显下降。结论合成后的P1肽具有良好的生物学活性;P1肽的作用靶点可能存在于炎症因子产生的上游信号通路中,P1肽不仅能从趋化因子mRNA水平降低趋化因子的表达量,同时还可抑制TNF-α的产生,进而发挥炎症抑制作用。

英文摘要：

To provide the rationale for the negative regulation of inflammatory reaction,we studied the action of the P1 peptide,which could significantly inhibit the expression of various types of inflammatory chemokines.After detecting the cytotoxicity of P1 peptide by MTT,we used some methods such as LUMINEX200,calcium flow test,RT-PCR test and ELISA to study the activity and function of P1 peptide.Finally it was found that P1 peptide could reduce the expression of both the chemokines and chemokines mRNA,but not through the way of TTP;and the secretion of inflammatory factor TNF-α was decreased by the action of P1 peptide compared with the LPS inflammatory model group.The results showed that P1 peptide possess biologic activity of can inhibiting the expression of inflammatory chemokines in mRNA level,and also inhibiting the secretion of TNF-α,then play the role of anti-inflammatory action.