中文摘要：

套作是防治连作障碍的有效方法之一,但是蔬菜和可食用菌之间的套作机理研究鲜见报道,尤其是其土壤微生物学机制。本研究建立菇菜套作体系,利用实时荧光定量PCR和PCR-DGGE技术研究土壤细菌和真菌群落的变化。结果表明,菇菜套作显著提高了番茄生物量,且其番茄果实产量最高,硝酸盐含量最低。与对照相比,菇菜套作下土壤细菌和真菌基因拷贝数量均无显著变化;DGGE指纹图谱表明,不同处理下的细菌群落无明显差异,但是菇菜套作下真菌群落结构发生了分异,主要表现为尖孢镰刀菌（Fusarium oxysporum）和稻黑孢菌（Nigrospora oryzae）代表型条带的强度的下降。

英文摘要：

Facility agriculture is an efficient way to solve the problem of food supply in China. However, it tends to lead to deterioration of soil health, such as the occurrence of continuous cropping obstacle, which in turn, triggers decline in yield and quality of the vegetables in facility agriculture obstacle. Numerous reports have been published on positive but few are available on effect of interplanting of vegetable nisms, such as soil microbiological mechanism. Interplanting is an effective way to avoid continuous cropping effects of interplanting of vegetables on vegetable production, with edible fungi, let alone papers on its underlying mecha- For this purpose, a tomato-Agaricus bisporus interplanting experiment was laid out. Tomato is a thermophilic and pho- tophilic species of plant while Agaricus bisporus prefers to grow in dark environment. Therefore the latter can flourish in the shade of the former. From the angle of gas exchange, tomatoes can get more CO2for photosynthesis from respiration of Agaricus bisporus, while Agaricus bisporus can have more 02 generated from tomato in photosynthesis to decompose cultural media into nutrients available to tomato for growth. It is, therefore, hypothesized that the relationship of mutual benefit be- tween tomato and Agaricus bisporus should contribute to improvement of crop yield and soil quality in facility agriculture. In the interplanting experiment, tomato seeds were sown in vermiculite for seedling culture. Thirty days later, the seedlings were transplanted into pots, 2 seedlings each planted in diagonally opposite corners and a ditch was dug in be- tween the two plants for culturing of Agaricus bisporus. The experiment was designed to have three treatments, each having three replicates; i.e. Treatment L （control without addition of any mushroom culturing media or inoculation of mushroom spores in the ditch） ; Treatment LS （mushroom culturing media added, but no mushroom spores inoculated） ; and Treat- ment LSA （mushroom culturing media added and mus